Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29086 | 5' | -59.7 | NC_006146.1 | + | 119043 | 0.66 | 0.724361 |
|
Target: 5'- -cAUCCcCAUCCCCGGGCucggGCCagGCc- -3' miRNA: 3'- uaUAGGcGUAGGGGUCCG----CGGa-CGua -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 126238 | 0.67 | 0.643975 |
|
Target: 5'- -cGUCCGUuuugggaCCCAGGCGCCcggGCu- -3' miRNA: 3'- uaUAGGCGuag----GGGUCCGCGGa--CGua -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 129911 | 0.66 | 0.714494 |
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Target: 5'- --cUCUGCGcCCUgGGGCGCCgGCGc -3' miRNA: 3'- uauAGGCGUaGGGgUCCGCGGaCGUa -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 139154 | 0.71 | 0.438753 |
|
Target: 5'- --cUCC-CGUCCCCcggggGGGCGCCUGUg- -3' miRNA: 3'- uauAGGcGUAGGGG-----UCCGCGGACGua -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 149909 | 0.66 | 0.694561 |
|
Target: 5'- -aGUCCGUGUCCCCGuagaugacucGuaGCCUGCc- -3' miRNA: 3'- uaUAGGCGUAGGGGU----------CcgCGGACGua -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 150714 | 0.66 | 0.714494 |
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Target: 5'- --cUCCuGCGcCCCCGGGgGCCcucUGCAa -3' miRNA: 3'- uauAGG-CGUaGGGGUCCgCGG---ACGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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