Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29086 | 5' | -59.7 | NC_006146.1 | + | 60712 | 0.67 | 0.674421 |
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Target: 5'- cAUGgggCCGCAUCUCCAaccGGCGCUgagGCc- -3' miRNA: 3'- -UAUa--GGCGUAGGGGU---CCGCGGa--CGua -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 71171 | 0.67 | 0.654144 |
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Target: 5'- -gGUCCGCGUCCUCGGaGCaCC-GCGUc -3' miRNA: 3'- uaUAGGCGUAGGGGUC-CGcGGaCGUA- -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 40059 | 0.67 | 0.643975 |
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Target: 5'- cGUGUCCGUaggacagcGUCUCCcGGCggaggGCCUGCAg -3' miRNA: 3'- -UAUAGGCG--------UAGGGGuCCG-----CGGACGUa -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 126238 | 0.67 | 0.643975 |
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Target: 5'- -cGUCCGUuuugggaCCCAGGCGCCcggGCu- -3' miRNA: 3'- uaUAGGCGuag----GGGUCCGCGGa--CGua -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 79055 | 0.73 | 0.339256 |
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Target: 5'- -gGUCCGaGUCCgCAGGCGCCggacGCAUg -3' miRNA: 3'- uaUAGGCgUAGGgGUCCGCGGa---CGUA- -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 114706 | 0.66 | 0.73415 |
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Target: 5'- -cGUCCGCGagaCCCGGGC-CCUGgCGg -3' miRNA: 3'- uaUAGGCGUag-GGGUCCGcGGAC-GUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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