Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
29093 | 3' | -56.6 | NC_006146.1 | + | 116557 | 0.67 | 0.83727 |
Target: 5'- -cCCUGCUCUUcacCCUGAGAUACAgCAc -3' miRNA: 3'- aaGGACGGGGA---GGACUCUGUGUaGUc -5' |
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29093 | 3' | -56.6 | NC_006146.1 | + | 88992 | 0.67 | 0.83727 |
Target: 5'- uUUCCUGaaaCUCUgCUGGGGCGCAUUu- -3' miRNA: 3'- -AAGGACg--GGGAgGACUCUGUGUAGuc -5' |
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29093 | 3' | -56.6 | NC_006146.1 | + | 124736 | 0.67 | 0.83727 |
Target: 5'- cUCUgGCCCCacccgaguUCCUGGGGCugGUCc- -3' miRNA: 3'- aAGGaCGGGG--------AGGACUCUGugUAGuc -5' |
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29093 | 3' | -56.6 | NC_006146.1 | + | 55663 | 0.66 | 0.861183 |
Target: 5'- aUCCcuggGCCUCUcagcugggcucCCUGGGugACAUCAu -3' miRNA: 3'- aAGGa---CGGGGA-----------GGACUCugUGUAGUc -5' |
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29093 | 3' | -56.6 | NC_006146.1 | + | 105832 | 0.66 | 0.87609 |
Target: 5'- cUCCUGCCCCgcgCCgccgaguAGACGCAg--- -3' miRNA: 3'- aAGGACGGGGa--GGac-----UCUGUGUaguc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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