Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29096 | 5' | -46.2 | NC_006146.1 | + | 166551 | 0.66 | 0.999964 |
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Target: 5'- ---cCGUGGCCAcGUcgCUGAA-GAGc -3' miRNA: 3'- auuuGCACCGGU-CAuaGACUUaCUUc -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 75060 | 0.66 | 0.999974 |
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Target: 5'- gGAGCcggGGCCAGUAcaggaUCUGGAgccGggGc -3' miRNA: 3'- aUUUGca-CCGGUCAU-----AGACUUa--CuuC- -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 33028 | 0.66 | 0.999974 |
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Target: 5'- aAAGCG-GGCUAGgcguaggGAGUGAGGg -3' miRNA: 3'- aUUUGCaCCGGUCauaga--CUUACUUC- -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 75108 | 0.66 | 0.999974 |
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Target: 5'- gGAACcggGGCCAGUAcaggaUCUGGAgccGggGc -3' miRNA: 3'- aUUUGca-CCGGUCAU-----AGACUUa--CuuC- -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 57341 | 0.66 | 0.999981 |
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Target: 5'- gGGGCGgGGCCccgGGUGUCg--GUGAGGg -3' miRNA: 3'- aUUUGCaCCGG---UCAUAGacuUACUUC- -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 109340 | 0.66 | 0.999981 |
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Target: 5'- -cAGCGUGGCCc---UCgGggUGggGa -3' miRNA: 3'- auUUGCACCGGucauAGaCuuACuuC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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