Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29096 | 5' | -46.2 | NC_006146.1 | + | 56395 | 0.67 | 0.99975 |
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Target: 5'- gUAAGCGUGGCCGcUcgCgaGggUGAGa -3' miRNA: 3'- -AUUUGCACCGGUcAuaGa-CuuACUUc -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 47670 | 0.68 | 0.999489 |
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Target: 5'- --cGCGgcGGCCAGgGUCucgUGGAUGGAGg -3' miRNA: 3'- auuUGCa-CCGGUCaUAG---ACUUACUUC- -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 46530 | 0.69 | 0.998797 |
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Target: 5'- ---cUGUGGCCGGUGUaggGAagGggGg -3' miRNA: 3'- auuuGCACCGGUCAUAga-CUuaCuuC- -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 44784 | 0.68 | 0.999489 |
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Target: 5'- gGAACccaGGCCGGggAUCUGGAUGGu- -3' miRNA: 3'- aUUUGca-CCGGUCa-UAGACUUACUuc -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 33028 | 0.66 | 0.999974 |
|
Target: 5'- aAAGCG-GGCUAGgcguaggGAGUGAGGg -3' miRNA: 3'- aUUUGCaCCGGUCauaga--CUUACUUC- -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 9679 | 0.67 | 0.999806 |
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Target: 5'- gAGAUGuUGGCCGugGUCaGGAUGAGGa -3' miRNA: 3'- aUUUGC-ACCGGUcaUAGaCUUACUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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