Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29096 | 5' | -46.2 | NC_006146.1 | + | 111642 | 0.69 | 0.998533 |
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Target: 5'- aAGACGUcGGCCAGgccCUGAacacccaggcccAUGGAGg -3' miRNA: 3'- aUUUGCA-CCGGUCauaGACU------------UACUUC- -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 103602 | 0.69 | 0.998533 |
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Target: 5'- cGGACGUGGCCcGGgagCUGGcgGAGc -3' miRNA: 3'- aUUUGCACCGG-UCauaGACUuaCUUc -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 92562 | 0.71 | 0.994097 |
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Target: 5'- aAAugGUGGCCAagugugccuGUAUCUGAAUu--- -3' miRNA: 3'- aUUugCACCGGU---------CAUAGACUUAcuuc -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 109377 | 0.71 | 0.992041 |
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Target: 5'- ---cCGUaGGCCAGguuUCUGAAUGAc- -3' miRNA: 3'- auuuGCA-CCGGUCau-AGACUUACUuc -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 159814 | 0.78 | 0.842909 |
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Target: 5'- gUAAACGUGGUgGGUAUCgcGggUGggGc -3' miRNA: 3'- -AUUUGCACCGgUCAUAGa-CuuACuuC- -5' |
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| 29096 | 5' | -46.2 | NC_006146.1 | + | 166551 | 0.66 | 0.999964 |
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Target: 5'- ---cCGUGGCCAcGUcgCUGAA-GAGc -3' miRNA: 3'- auuuGCACCGGU-CAuaGACUUaCUUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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