Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29104 | 5' | -55.2 | NC_006146.1 | + | 138072 | 0.69 | 0.825945 |
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Target: 5'- --gGCCGCCGCAAggacgccGG-GCcGGCUGGg -3' miRNA: 3'- cggUGGCGGCGUUa------CCaCGuUCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 148422 | 0.69 | 0.817333 |
|
Target: 5'- uGCCACugaggaaggCGCUGUAugaucUGGUGCAGGCa-- -3' miRNA: 3'- -CGGUG---------GCGGCGUu----ACCACGUUCGauc -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 148543 | 0.67 | 0.894567 |
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Target: 5'- gGCCACUGCCaUGAUgGGUGguGGCcGGu -3' miRNA: 3'- -CGGUGGCGGcGUUA-CCACguUCGaUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 154657 | 0.71 | 0.693095 |
|
Target: 5'- uGCCGCCgGCUGCAccGUGGUGgCGGggggcgcugcuGCUGGg -3' miRNA: 3'- -CGGUGG-CGGCGU--UACCAC-GUU-----------CGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 159929 | 0.69 | 0.808551 |
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Target: 5'- -gCGCCGCCuGCuuuaGGUGCAcuguggcaGGCUGGg -3' miRNA: 3'- cgGUGGCGG-CGuua-CCACGU--------UCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 164387 | 0.69 | 0.825945 |
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Target: 5'- uGCCGCCGCCGCcaagGGU-CAggAGCa-- -3' miRNA: 3'- -CGGUGGCGGCGuua-CCAcGU--UCGauc -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 168092 | 0.68 | 0.858531 |
|
Target: 5'- cGCCACCGCCuuGGucUGGaagGCcuGCUGGc -3' miRNA: 3'- -CGGUGGCGGcgUU--ACCa--CGuuCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 170102 | 0.71 | 0.693095 |
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Target: 5'- gGCCGgCGCCGCAggGGggGCcGGCgGGg -3' miRNA: 3'- -CGGUgGCGGCGUuaCCa-CGuUCGaUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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