Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29104 | 5' | -55.2 | NC_006146.1 | + | 34242 | 0.69 | 0.825945 |
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Target: 5'- --gGCCGCCGCAAggacgccGG-GCcGGCUGGg -3' miRNA: 3'- cggUGGCGGCGUUa------CCaCGuUCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 33260 | 0.66 | 0.94008 |
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Target: 5'- gGCCGCUGCCccGCuccgGGUGgGggguggcccGGCUGGg -3' miRNA: 3'- -CGGUGGCGG--CGuua-CCACgU---------UCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 33136 | 0.66 | 0.94008 |
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Target: 5'- gGCCGCUGCCccGCuccgGGUGgGggguggcccGGCUGGg -3' miRNA: 3'- -CGGUGGCGG--CGuua-CCACgU---------UCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 33001 | 0.76 | 0.443685 |
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Target: 5'- uGCCGCCGCUacccccggGgGAUGGUuaaaGCGGGCUAGg -3' miRNA: 3'- -CGGUGGCGG--------CgUUACCA----CGUUCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 19738 | 0.71 | 0.693095 |
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Target: 5'- cGCUACCGCCGCAucc-UGCugacGCUGGg -3' miRNA: 3'- -CGGUGGCGGCGUuaccACGuu--CGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 11487 | 0.67 | 0.887808 |
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Target: 5'- aGCCACCcggacucggggGCCGuCAugaucGUGGacagcgcggccUGCGAGCUGGc -3' miRNA: 3'- -CGGUGG-----------CGGC-GU-----UACC-----------ACGUUCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 11371 | 0.67 | 0.887808 |
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Target: 5'- cGCUGCgCGUCagGCAgGUGG-GCAAGCUGGu -3' miRNA: 3'- -CGGUG-GCGG--CGU-UACCaCGUUCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 4895 | 0.73 | 0.590431 |
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Target: 5'- gGCCugCGCCGCc-UGGUGacaGAGCcGGa -3' miRNA: 3'- -CGGugGCGGCGuuACCACg--UUCGaUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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