Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29104 | 5' | -55.2 | NC_006146.1 | + | 55190 | 0.7 | 0.752759 |
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Target: 5'- gGCUGCgGCCGCGGcGG-GCGAGCg-- -3' miRNA: 3'- -CGGUGgCGGCGUUaCCaCGUUCGauc -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 55960 | 0.7 | 0.771886 |
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Target: 5'- cGCCAUCGCCGCccaggGGUaGC-GGUUGGu -3' miRNA: 3'- -CGGUGGCGGCGuua--CCA-CGuUCGAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 116493 | 0.69 | 0.799606 |
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Target: 5'- uCCACCcccagGCCGCAcaugugggcguUGGUGCAGGCcGGu -3' miRNA: 3'- cGGUGG-----CGGCGUu----------ACCACGUUCGaUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 47809 | 0.69 | 0.799606 |
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Target: 5'- cCCACCGCCGUcucAUGGgcuCGGGCUGc -3' miRNA: 3'- cGGUGGCGGCGu--UACCac-GUUCGAUc -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 53041 | 0.69 | 0.799606 |
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Target: 5'- gGCCGCCGCCGagaGGUaaGGgggGCGGGUgGGg -3' miRNA: 3'- -CGGUGGCGGCg--UUA--CCa--CGUUCGaUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 148422 | 0.69 | 0.817333 |
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Target: 5'- uGCCACugaggaaggCGCUGUAugaucUGGUGCAGGCa-- -3' miRNA: 3'- -CGGUG---------GCGGCGUu----ACCACGUUCGauc -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 44821 | 0.69 | 0.817333 |
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Target: 5'- gGCC-CCGCUGCGGcUGGUGacgGAGgUGGg -3' miRNA: 3'- -CGGuGGCGGCGUU-ACCACg--UUCgAUC- -5' |
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| 29104 | 5' | -55.2 | NC_006146.1 | + | 129452 | 1.1 | 0.00311 |
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Target: 5'- uGCCACCGCCGCAAUGGUGCAAGCUAGc -3' miRNA: 3'- -CGGUGGCGGCGUUACCACGUUCGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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