Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
29107 | 5' | -58.4 | NC_006146.1 | + | 144731 | 0.69 | 0.668524 |
Target: 5'- gACGCUGgcacacCGGGC-CGCCGGGGUc -3' miRNA: 3'- -UGCGACaggau-GCCUGuGCGGCCUCA- -5' |
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29107 | 5' | -58.4 | NC_006146.1 | + | 141654 | 0.69 | 0.668524 |
Target: 5'- gACGCUGgcacacCGGGC-CGCCGGGGUc -3' miRNA: 3'- -UGCGACaggau-GCCUGuGCGGCCUCA- -5' |
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29107 | 5' | -58.4 | NC_006146.1 | + | 30215 | 0.7 | 0.628688 |
Target: 5'- cCGCUGcgcCCgcCGGGCugGCCGGAu- -3' miRNA: 3'- uGCGACa--GGauGCCUGugCGGCCUca -5' |
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29107 | 5' | -58.4 | NC_006146.1 | + | 99992 | 0.7 | 0.618716 |
Target: 5'- uCGC-GUCCUcgggaucgggaGCGGGCACGUCgGGAGg -3' miRNA: 3'- uGCGaCAGGA-----------UGCCUGUGCGG-CCUCa -5' |
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29107 | 5' | -58.4 | NC_006146.1 | + | 58414 | 0.71 | 0.559325 |
Target: 5'- cCGCUaUCuCUAUGaGCGCGCCGGGGUg -3' miRNA: 3'- uGCGAcAG-GAUGCcUGUGCGGCCUCA- -5' |
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29107 | 5' | -58.4 | NC_006146.1 | + | 6652 | 0.72 | 0.530193 |
Target: 5'- uACGCUGcCCUaGCGGuuAUGCUGGGGa -3' miRNA: 3'- -UGCGACaGGA-UGCCugUGCGGCCUCa -5' |
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29107 | 5' | -58.4 | NC_006146.1 | + | 130848 | 1.06 | 0.003155 |
Target: 5'- gACGCUGUCCUACGGACACGCCGGAGUc -3' miRNA: 3'- -UGCGACAGGAUGCCUGUGCGGCCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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