Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29107 | 5' | -58.4 | NC_006146.1 | + | 2318 | 0.67 | 0.809546 |
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Target: 5'- cACGCccccggGUCCccCGGcACGCGCCGGGc- -3' miRNA: 3'- -UGCGa-----CAGGauGCC-UGUGCGGCCUca -5' |
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| 29107 | 5' | -58.4 | NC_006146.1 | + | 1386 | 0.67 | 0.809546 |
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Target: 5'- cACGCccccggGUCCccCGGcACGCGCCGGGc- -3' miRNA: 3'- -UGCGa-----CAGGauGCC-UGUGCGGCCUca -5' |
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| 29107 | 5' | -58.4 | NC_006146.1 | + | 455 | 0.67 | 0.809546 |
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Target: 5'- cACGCccccggGUCCccCGGcACGCGCCGGGc- -3' miRNA: 3'- -UGCGa-----CAGGauGCC-UGUGCGGCCUca -5' |
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| 29107 | 5' | -58.4 | NC_006146.1 | + | 64463 | 0.67 | 0.764984 |
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Target: 5'- gGCGCUGUCCUGCugaaGGGCAUuUCuGAGg -3' miRNA: 3'- -UGCGACAGGAUG----CCUGUGcGGcCUCa -5' |
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| 29107 | 5' | -58.4 | NC_006146.1 | + | 157043 | 0.69 | 0.668524 |
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Target: 5'- gACGCUGgcacacCGGGC-CGCCGGGGUc -3' miRNA: 3'- -UGCGACaggau-GCCUGuGCGGCCUCA- -5' |
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| 29107 | 5' | -58.4 | NC_006146.1 | + | 153965 | 0.69 | 0.668524 |
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Target: 5'- gACGCUGgcacacCGGGC-CGCCGGGGUc -3' miRNA: 3'- -UGCGACaggau-GCCUGuGCGGCCUCA- -5' |
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| 29107 | 5' | -58.4 | NC_006146.1 | + | 63822 | 0.66 | 0.850298 |
|
Target: 5'- cACGacuauccagGUCUUGCcGugGCGCCGGGGa -3' miRNA: 3'- -UGCga-------CAGGAUGcCugUGCGGCCUCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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