Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29108 | 5' | -58.1 | NC_006146.1 | + | 93271 | 0.66 | 0.86237 |
Target: 5'- aGCCgCUCGCGggguugcCCUCGCCGguggucaUCUCUGa-- -3' miRNA: 3'- -UGG-GAGCGU-------GGAGCGGC-------AGAGAUgac -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 47795 | 0.66 | 0.848666 |
Target: 5'- aGCCCcgucuaGCGCCcacCGCCGUCUCauggGCUc -3' miRNA: 3'- -UGGGag----CGUGGa--GCGGCAGAGa---UGAc -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 4922 | 0.66 | 0.840788 |
Target: 5'- gACCCUCGCugUcgggUgGCCGUgCUCUGu-- -3' miRNA: 3'- -UGGGAGCGugG----AgCGGCA-GAGAUgac -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 130833 | 0.67 | 0.816088 |
Target: 5'- gGCCCUC-CGCCgggagaCGCUGUC-CUACg- -3' miRNA: 3'- -UGGGAGcGUGGa-----GCGGCAGaGAUGac -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 107159 | 0.67 | 0.789946 |
Target: 5'- cGCCCaccagaaacaCGuCGCCUcCGCCGUCUUcACUGa -3' miRNA: 3'- -UGGGa---------GC-GUGGA-GCGGCAGAGaUGAC- -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 160180 | 0.67 | 0.788158 |
Target: 5'- gGCUCUgGCugcccagauuauCCUCGCCaGUCUgUGCUGc -3' miRNA: 3'- -UGGGAgCGu-----------GGAGCGG-CAGAgAUGAC- -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 91204 | 0.67 | 0.780949 |
Target: 5'- cCCCcCGCcuGCCUguugGCCgGUCUCUGCUGu -3' miRNA: 3'- uGGGaGCG--UGGAg---CGG-CAGAGAUGAC- -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 48304 | 0.67 | 0.771824 |
Target: 5'- cCCCUCGC-CCUCcaCCGUCggaggUGCUGg -3' miRNA: 3'- uGGGAGCGuGGAGc-GGCAGag---AUGAC- -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 110393 | 0.68 | 0.76258 |
Target: 5'- gGCCCagugggaGCAcCCUCGCCGUCgggggaGCUGg -3' miRNA: 3'- -UGGGag-----CGU-GGAGCGGCAGaga---UGAC- -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 127898 | 0.7 | 0.655462 |
Target: 5'- aGCCCUCGCACCUCucacCCGgcggcaaUUCUACc- -3' miRNA: 3'- -UGGGAGCGUGGAGc---GGCa------GAGAUGac -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 122884 | 0.7 | 0.605385 |
Target: 5'- -gCCUCGCACCUC-CCGg--UUGCUGg -3' miRNA: 3'- ugGGAGCGUGGAGcGGCagaGAUGAC- -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 63287 | 0.71 | 0.585438 |
Target: 5'- aGCCCUgGCACCUCcgguauCUGUCccugUCUGCUGc -3' miRNA: 3'- -UGGGAgCGUGGAGc-----GGCAG----AGAUGAC- -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 41658 | 0.82 | 0.146343 |
Target: 5'- gACCCUCGCGCCUCaccugggcCCGUCcCUGCUGg -3' miRNA: 3'- -UGGGAGCGUGGAGc-------GGCAGaGAUGAC- -5' |
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29108 | 5' | -58.1 | NC_006146.1 | + | 133017 | 1.07 | 0.002879 |
Target: 5'- gACCCUCGCACCUCGCCGUCUCUACUGc -3' miRNA: 3'- -UGGGAGCGUGGAGCGGCAGAGAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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