Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29110 | 3' | -46.6 | NC_006146.1 | + | 133735 | 1.11 | 0.021189 |
Target: 5'- gCUUCGAGGUAAACAUCGGCUUACUGAa -3' miRNA: 3'- -GAAGCUCCAUUUGUAGCCGAAUGACU- -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 111352 | 0.72 | 0.98844 |
Target: 5'- --cCGAGGUGGGCAUCagGGCgacaaACUGGg -3' miRNA: 3'- gaaGCUCCAUUUGUAG--CCGaa---UGACU- -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 146217 | 0.7 | 0.997021 |
Target: 5'- --cCGGGGUAAGCggCGGCcaugGCUGc -3' miRNA: 3'- gaaGCUCCAUUUGuaGCCGaa--UGACu -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 128499 | 0.69 | 0.998261 |
Target: 5'- cCUUCGAGGacGGCGUCGGggUGgccCUGGg -3' miRNA: 3'- -GAAGCUCCauUUGUAGCCgaAU---GACU- -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 135893 | 0.68 | 0.999366 |
Target: 5'- --gCGuGGcGAGCAUCGGUcaGCUGAc -3' miRNA: 3'- gaaGCuCCaUUUGUAGCCGaaUGACU- -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 32063 | 0.68 | 0.999366 |
Target: 5'- --gCGuGGcGAGCAUCGGUcaGCUGAc -3' miRNA: 3'- gaaGCuCCaUUUGUAGCCGaaUGACU- -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 56853 | 0.68 | 0.999492 |
Target: 5'- --gCGGGGgcGGCAgcuUUGGCUgggGCUGGc -3' miRNA: 3'- gaaGCUCCauUUGU---AGCCGAa--UGACU- -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 63082 | 0.68 | 0.999492 |
Target: 5'- -cUCGGGGgGAACAUgCGGUggccucGCUGAa -3' miRNA: 3'- gaAGCUCCaUUUGUA-GCCGaa----UGACU- -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 115421 | 0.68 | 0.999595 |
Target: 5'- --cCGGGGUAAcuauaaucucACAggUGGCUUGCUGc -3' miRNA: 3'- gaaGCUCCAUU----------UGUa-GCCGAAUGACu -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 36236 | 0.66 | 0.999972 |
Target: 5'- --cCGGGGaggAGGCAgggCGGCUU-CUGGa -3' miRNA: 3'- gaaGCUCCa--UUUGUa--GCCGAAuGACU- -5' |
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29110 | 3' | -46.6 | NC_006146.1 | + | 63768 | 0.66 | 0.999932 |
Target: 5'- --cUGAGGUGAACAccguUCuGCUUGuCUGGg -3' miRNA: 3'- gaaGCUCCAUUUGU----AGcCGAAU-GACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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