Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29110 | 5' | -54.6 | NC_006146.1 | + | 126185 | 0.66 | 0.957529 |
Target: 5'- gGGcCAGUGGGCaggcacCUGauagagUCCCUCAUc- -3' miRNA: 3'- -CCaGUCACCCG------GACaa----AGGGAGUGuc -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 129111 | 0.66 | 0.956777 |
Target: 5'- cGG-CAGaGGGCCUGagagcgggcgCCCgggCGCAGg -3' miRNA: 3'- -CCaGUCaCCCGGACaaa-------GGGa--GUGUC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 52091 | 0.66 | 0.953677 |
Target: 5'- cGGUCGG-GGGCCggcgaggccaucUGUUcCCCgUCGCuGg -3' miRNA: 3'- -CCAGUCaCCCGG------------ACAAaGGG-AGUGuC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 168496 | 0.66 | 0.951256 |
Target: 5'- cGGUCGGgGGGCCgGggaccaacagagCCCUCugGa -3' miRNA: 3'- -CCAGUCaCCCGGaCaaa---------GGGAGugUc -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 107102 | 0.66 | 0.949596 |
Target: 5'- aGUgGGUGGGUCUGggaaggggCCUUCACc- -3' miRNA: 3'- cCAgUCACCCGGACaaa-----GGGAGUGuc -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 128059 | 0.66 | 0.945282 |
Target: 5'- ----cGUGGGCCUGgcggCCCUCGa-- -3' miRNA: 3'- ccaguCACCCGGACaaa-GGGAGUguc -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 164247 | 0.66 | 0.944838 |
Target: 5'- -aUCAGUgGGGCCUGgugggccaccUCCCUCGuugcuuuCAGg -3' miRNA: 3'- ccAGUCA-CCCGGACaa--------AGGGAGU-------GUC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 82516 | 0.66 | 0.944838 |
Target: 5'- uGGUCAG-GGccugcacGCCUGguaauaucagccUUUCCCUgGCAGc -3' miRNA: 3'- -CCAGUCaCC-------CGGAC------------AAAGGGAgUGUC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 104690 | 0.67 | 0.940733 |
Target: 5'- aGGggCAGcUGGGCCUGgaUCUCgUCGCc- -3' miRNA: 3'- -CCa-GUC-ACCCGGACaaAGGG-AGUGuc -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 112519 | 0.67 | 0.935946 |
Target: 5'- gGGcCGGUggaGGGCCUGggUCaCCUCGa-- -3' miRNA: 3'- -CCaGUCA---CCCGGACaaAG-GGAGUguc -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 109415 | 0.67 | 0.925656 |
Target: 5'- cGUCGGUcaGGGCCcGUUUggccCCCUCAaaaAGa -3' miRNA: 3'- cCAGUCA--CCCGGaCAAA----GGGAGUg--UC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 137778 | 0.67 | 0.920151 |
Target: 5'- cGGUCcgGGUGGGCCUGg--UCCgC-CGGa -3' miRNA: 3'- -CCAG--UCACCCGGACaaaGGGaGuGUC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 126628 | 0.67 | 0.914406 |
Target: 5'- uGGUCGGUGGGCacac--CCCggggccCGCAGg -3' miRNA: 3'- -CCAGUCACCCGgacaaaGGGa-----GUGUC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 71650 | 0.67 | 0.914406 |
Target: 5'- uGGUUcuuGGUGGGCUUGUUaaUCCUgagC-CAGg -3' miRNA: 3'- -CCAG---UCACCCGGACAA--AGGGa--GuGUC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 36477 | 0.67 | 0.914406 |
Target: 5'- gGGUCAuUGGGCCUGUUgaaaaguaaaCCg-GCAGg -3' miRNA: 3'- -CCAGUcACCCGGACAAag--------GGagUGUC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 159260 | 0.68 | 0.90472 |
Target: 5'- -aUCGGUgcaacgccgccacgcGGGCCUcGgggCCCUCGCAGu -3' miRNA: 3'- ccAGUCA---------------CCCGGA-CaaaGGGAGUGUC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 121565 | 0.73 | 0.642077 |
Target: 5'- aGGUgaaaAGUGGGCCUGagUcucacauuaaagccCCCUCACAGc -3' miRNA: 3'- -CCAg---UCACCCGGACaaA--------------GGGAGUGUC- -5' |
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29110 | 5' | -54.6 | NC_006146.1 | + | 133774 | 1.12 | 0.002971 |
Target: 5'- aGGUCAGUGGGCCUGUUUCCCUCACAGa -3' miRNA: 3'- -CCAGUCACCCGGACAAAGGGAGUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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