Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29113 | 5' | -59.1 | NC_006146.1 | + | 127122 | 0.66 | 0.78712 |
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Target: 5'- cGCCCggaGAccUG-GUGGUCCCCUCUg -3' miRNA: 3'- uCGGGaagCU--ACgUACCAGGGGGGAa -5' |
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| 29113 | 5' | -59.1 | NC_006146.1 | + | 61911 | 0.66 | 0.777997 |
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Target: 5'- aAGCCCc-UGA-GCGUgGGUCCCUCCg- -3' miRNA: 3'- -UCGGGaaGCUaCGUA-CCAGGGGGGaa -5' |
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| 29113 | 5' | -59.1 | NC_006146.1 | + | 141157 | 0.66 | 0.768748 |
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Target: 5'- cAGCagCUgCGGUGguUGGUUCCCUCUUu -3' miRNA: 3'- -UCGg-GAaGCUACguACCAGGGGGGAA- -5' |
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| 29113 | 5' | -59.1 | NC_006146.1 | + | 120885 | 0.68 | 0.661109 |
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Target: 5'- gGGcCCCUUC--UGCuucgGUGGUgCCCCCCUUc -3' miRNA: 3'- -UC-GGGAAGcuACG----UACCA-GGGGGGAA- -5' |
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| 29113 | 5' | -59.1 | NC_006146.1 | + | 140751 | 0.68 | 0.651016 |
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Target: 5'- cGCUCUcgGAUGCGUGGccaugcccaCCCCCCUg -3' miRNA: 3'- uCGGGAagCUACGUACCa--------GGGGGGAa -5' |
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| 29113 | 5' | -59.1 | NC_006146.1 | + | 56664 | 0.69 | 0.62068 |
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Target: 5'- uGGCCCUcccugaGGUGCcgGG-CCCCCUg- -3' miRNA: 3'- -UCGGGAag----CUACGuaCCaGGGGGGaa -5' |
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| 29113 | 5' | -59.1 | NC_006146.1 | + | 52900 | 0.69 | 0.600486 |
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Target: 5'- gAGCCCcgcaUGGucUGCGUGG-CCCCCCg- -3' miRNA: 3'- -UCGGGaa--GCU--ACGUACCaGGGGGGaa -5' |
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| 29113 | 5' | -59.1 | NC_006146.1 | + | 132714 | 0.73 | 0.385827 |
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Target: 5'- gGGCCCUUcaCGAUGCccGGUgUCCCCa- -3' miRNA: 3'- -UCGGGAA--GCUACGuaCCAgGGGGGaa -5' |
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| 29113 | 5' | -59.1 | NC_006146.1 | + | 134203 | 1.05 | 0.002414 |
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Target: 5'- cAGCCCUUCGAUGCAUGGUCCCCCCUUa -3' miRNA: 3'- -UCGGGAAGCUACGUACCAGGGGGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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