Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29132 | 3' | -59.2 | NC_006146.1 | + | 163746 | 0.66 | 0.773735 |
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Target: 5'- aGGUGUGUGgGCCUUaGUGGGGc---- -3' miRNA: 3'- aCCGCGCACgCGGAA-CACUCCcacua -5' |
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| 29132 | 3' | -59.2 | NC_006146.1 | + | 40452 | 0.66 | 0.772812 |
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Target: 5'- aGGCGgGUGCggcaaugGCCUg--GuGGGUGAa -3' miRNA: 3'- aCCGCgCACG-------CGGAacaCuCCCACUa -5' |
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| 29132 | 3' | -59.2 | NC_006146.1 | + | 157272 | 0.66 | 0.764449 |
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Target: 5'- cGGCGCuUGaucgucaGCCccaggGUGGGGGUGGc -3' miRNA: 3'- aCCGCGcACg------CGGaa---CACUCCCACUa -5' |
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| 29132 | 3' | -59.2 | NC_006146.1 | + | 161673 | 0.66 | 0.745543 |
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Target: 5'- gUGGUGCGUGUgugugcuaauugGCCgugUGUGAuGGG-GAg -3' miRNA: 3'- -ACCGCGCACG------------CGGa--ACACU-CCCaCUa -5' |
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| 29132 | 3' | -59.2 | NC_006146.1 | + | 163911 | 0.67 | 0.726251 |
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Target: 5'- aGGaugaaGCGUGgGCCUUGuUGGGGGc--- -3' miRNA: 3'- aCCg----CGCACgCGGAAC-ACUCCCacua -5' |
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| 29132 | 3' | -59.2 | NC_006146.1 | + | 139358 | 0.67 | 0.696743 |
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Target: 5'- aGGgGCG-GCGCCggG-GGGGGUGc- -3' miRNA: 3'- aCCgCGCaCGCGGaaCaCUCCCACua -5' |
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| 29132 | 3' | -59.2 | NC_006146.1 | + | 53470 | 0.67 | 0.68679 |
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Target: 5'- aGGCGCGgGUGCCUcGUcggucagggccGGGGGUGc- -3' miRNA: 3'- aCCGCGCaCGCGGAaCA-----------CUCCCACua -5' |
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| 29132 | 3' | -59.2 | NC_006146.1 | + | 161444 | 0.69 | 0.566214 |
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Target: 5'- gGGCGCGgaccccGCGCCcaccGUGAcacaGGGUGAa -3' miRNA: 3'- aCCGCGCa-----CGCGGaa--CACU----CCCACUa -5' |
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| 29132 | 3' | -59.2 | NC_006146.1 | + | 163692 | 0.73 | 0.366633 |
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Target: 5'- aGGCGCGUGgGCCUUG-GuGGGg--- -3' miRNA: 3'- aCCGCGCACgCGGAACaCuCCCacua -5' |
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| 29132 | 3' | -59.2 | NC_006146.1 | + | 152487 | 1.06 | 0.002287 |
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Target: 5'- cUGGCGCGUGCGCCUUGUGAGGGUGAUg -3' miRNA: 3'- -ACCGCGCACGCGGAACACUCCCACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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