Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29133 | 3' | -59.3 | NC_006146.1 | + | 31959 | 0.66 | 0.753224 |
|
Target: 5'- --aGCCaGGGCCAGGUUAGGccaAGaGUCu -3' miRNA: 3'- cuaCGG-CCCGGUCUAGUCCc--UC-CAGu -5' |
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| 29133 | 3' | -59.3 | NC_006146.1 | + | 28276 | 0.69 | 0.575509 |
|
Target: 5'- --gGCCGGGUCucGGGUCuGGG-GGUCu -3' miRNA: 3'- cuaCGGCCCGG--UCUAGuCCCuCCAGu -5' |
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| 29133 | 3' | -59.3 | NC_006146.1 | + | 25198 | 0.69 | 0.575509 |
|
Target: 5'- --gGCCGGGUCucGGGUCuGGG-GGUCu -3' miRNA: 3'- cuaCGGCCCGG--UCUAGuCCCuCCAGu -5' |
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| 29133 | 3' | -59.3 | NC_006146.1 | + | 22120 | 0.69 | 0.575509 |
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Target: 5'- --gGCCGGGUCucGGGUCuGGG-GGUCu -3' miRNA: 3'- cuaCGGCCCGG--UCUAGuCCCuCCAGu -5' |
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| 29133 | 3' | -59.3 | NC_006146.1 | + | 19042 | 0.69 | 0.575509 |
|
Target: 5'- --gGCCGGGUCucGGGUCuGGG-GGUCu -3' miRNA: 3'- cuaCGGCCCGG--UCUAGuCCCuCCAGu -5' |
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| 29133 | 3' | -59.3 | NC_006146.1 | + | 14816 | 0.75 | 0.301145 |
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Target: 5'- --gGCCGGGCCGGAggAGGGcacGGUCu -3' miRNA: 3'- cuaCGGCCCGGUCUagUCCCu--CCAGu -5' |
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| 29133 | 3' | -59.3 | NC_006146.1 | + | 12887 | 0.69 | 0.575509 |
|
Target: 5'- --gGCCGGGUCucGGGUCuGGG-GGUCu -3' miRNA: 3'- cuaCGGCCCGG--UCUAGuCCCuCCAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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