Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29135 | 5' | -57.6 | NC_006146.1 | + | 67330 | 0.69 | 0.704046 |
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Target: 5'- gAGCCCgg-CCCagGCCCGggcGAGCCc -3' miRNA: 3'- -UCGGGagaGGGaaCGGGCaa-CUUGGc -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 9799 | 0.7 | 0.644139 |
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Target: 5'- uGCuCCUCUCCaggGCCCaGUUuGAACCa -3' miRNA: 3'- uCG-GGAGAGGgaaCGGG-CAA-CUUGGc -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 88565 | 0.71 | 0.624011 |
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Target: 5'- gGGCCCUgaCUCCCggGCUCuUUGAACUa -3' miRNA: 3'- -UCGGGA--GAGGGaaCGGGcAACUUGGc -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 170559 | 0.71 | 0.613952 |
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Target: 5'- cGCCCUCcCUCUgcuCCCGUUG-GCCGg -3' miRNA: 3'- uCGGGAGaGGGAac-GGGCAACuUGGC- -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 126147 | 0.71 | 0.583883 |
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Target: 5'- -aCCCUCUCCCccgagaGCCCGauugGAGCCa -3' miRNA: 3'- ucGGGAGAGGGaa----CGGGCaa--CUUGGc -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 53912 | 0.71 | 0.583883 |
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Target: 5'- gGGCCCgCUCCaCgcGCUCGUUGAGCUc -3' miRNA: 3'- -UCGGGaGAGG-GaaCGGGCAACUUGGc -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 47854 | 0.72 | 0.515172 |
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Target: 5'- aGGaCCCUCUCCUccagcGCCCGgaccUGGGCCGc -3' miRNA: 3'- -UC-GGGAGAGGGaa---CGGGCa---ACUUGGC- -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 116257 | 0.79 | 0.215203 |
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Target: 5'- gGGCCC-CUCCaaUGCCCGggGAGCCa -3' miRNA: 3'- -UCGGGaGAGGgaACGGGCaaCUUGGc -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 155929 | 1.09 | 0.002311 |
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Target: 5'- cAGCCCUCUCCCUUGCCCGUUGAACCGa -3' miRNA: 3'- -UCGGGAGAGGGAACGGGCAACUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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