Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29139 | 3' | -54.6 | NC_006146.1 | + | 21343 | 0.66 | 0.950688 |
Target: 5'- gUCaUUCUC--GGAgGGGCUgCCGGCg -3' miRNA: 3'- aAGaAAGAGuaCCUaCUCGGgGGCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 64913 | 0.66 | 0.946382 |
Target: 5'- ------gUCGUGGAUGGGCUCCagGGUu -3' miRNA: 3'- aagaaagAGUACCUACUCGGGGg-CCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 155013 | 0.66 | 0.932011 |
Target: 5'- -gCUggCUCGaGGccagcGAGCCCuCCGGCa -3' miRNA: 3'- aaGAaaGAGUaCCua---CUCGGG-GGCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 37229 | 0.67 | 0.92455 |
Target: 5'- cUCUUUCUCAUaucccaaaaAGCuCCCCGGCc -3' miRNA: 3'- aAGAAAGAGUAccuac----UCG-GGGGCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 82478 | 0.67 | 0.921205 |
Target: 5'- ----cUCUUccGGaAUGAcCCCCCGGCa -3' miRNA: 3'- aagaaAGAGuaCC-UACUcGGGGGCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 146593 | 0.68 | 0.889926 |
Target: 5'- gUCgcgCUCuggGUGGAgggGAaacagcguccaGCCCCCGGCc -3' miRNA: 3'- aAGaaaGAG---UACCUa--CU-----------CGGGGGCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 67074 | 0.68 | 0.882957 |
Target: 5'- cUC-UUCUCccagguuugGUGGcgGAGCUCuuGGCa -3' miRNA: 3'- aAGaAAGAG---------UACCuaCUCGGGggCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 57334 | 0.68 | 0.882957 |
Target: 5'- ----cUCUCugggGGcgGGGCCCCgGGUg -3' miRNA: 3'- aagaaAGAGua--CCuaCUCGGGGgCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 65170 | 0.68 | 0.882957 |
Target: 5'- cUCUUggagCUCuUGGGgcucuugGAGCCCuaGGCg -3' miRNA: 3'- aAGAAa---GAGuACCUa------CUCGGGggCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 141307 | 0.68 | 0.860699 |
Target: 5'- -----gUUCAgccUGGcUGAGCCCCCGGg -3' miRNA: 3'- aagaaaGAGU---ACCuACUCGGGGGCCg -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 150273 | 0.69 | 0.836533 |
Target: 5'- cUUCUUUacaaaGUGGuagGuGCCCCCGGUg -3' miRNA: 3'- -AAGAAAgag--UACCua-CuCGGGGGCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 157772 | 0.69 | 0.819455 |
Target: 5'- cUCUc-CUCGUGGGcccggGAGCCCCCGu- -3' miRNA: 3'- aAGAaaGAGUACCUa----CUCGGGGGCcg -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 122765 | 0.72 | 0.694621 |
Target: 5'- aUCUUggggaUC-UGGcgGGGCuCCCCGGCg -3' miRNA: 3'- aAGAAag---AGuACCuaCUCG-GGGGCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 145079 | 0.77 | 0.407489 |
Target: 5'- aUCUc-CUCAUgagggugccgagGGAUGGGUCCCCGGCg -3' miRNA: 3'- aAGAaaGAGUA------------CCUACUCGGGGGCCG- -5' |
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29139 | 3' | -54.6 | NC_006146.1 | + | 157316 | 1.08 | 0.004194 |
Target: 5'- cUUCUUUCUCAUGGAUGAGCCCCCGGCc -3' miRNA: 3'- -AAGAAAGAGUACCUACUCGGGGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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