Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29140 | 3' | -51.5 | NC_006146.1 | + | 108579 | 0.66 | 0.994363 |
Target: 5'- uGGCgGCgGCGUCUcUGGCcucgcuggGCUCUCc -3' miRNA: 3'- -CCG-UGgUGCAGAuACUGcaa-----CGAGAG- -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 74330 | 0.66 | 0.994363 |
Target: 5'- -cCACCACGUCaGUGACcccacugaugGUcuUGUUCUCc -3' miRNA: 3'- ccGUGGUGCAGaUACUG----------CA--ACGAGAG- -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 50090 | 0.66 | 0.990158 |
Target: 5'- cGGCACCugGaUCU--GACuccgGUUGCggagCUCg -3' miRNA: 3'- -CCGUGGugC-AGAuaCUG----CAACGa---GAG- -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 140632 | 0.66 | 0.989357 |
Target: 5'- gGGgACCACGgcgcuccuuggugcCUGUGGUGaUGCUCUCu -3' miRNA: 3'- -CCgUGGUGCa-------------GAUACUGCaACGAGAG- -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 123791 | 0.66 | 0.991387 |
Target: 5'- uGGCugC-CGUCaugGUGACG--GCUCUg -3' miRNA: 3'- -CCGugGuGCAGa--UACUGCaaCGAGAg -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 144838 | 0.66 | 0.993574 |
Target: 5'- cGGC-CCACGUUgc-GGCGggccaauugcagcgGCUCUCc -3' miRNA: 3'- -CCGuGGUGCAGauaCUGCaa------------CGAGAG- -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 120189 | 0.67 | 0.979671 |
Target: 5'- gGGC-CCuCGUCUAUGACccgGC-CUCu -3' miRNA: 3'- -CCGuGGuGCAGAUACUGcaaCGaGAG- -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 165917 | 0.68 | 0.977322 |
Target: 5'- aGGCACCccGCGUUaGUGAaggccgccaGgagGCUCUCg -3' miRNA: 3'- -CCGUGG--UGCAGaUACUg--------Caa-CGAGAG- -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 101860 | 0.68 | 0.977322 |
Target: 5'- cGCACCACGg--GUGGCGggGCg--- -3' miRNA: 3'- cCGUGGUGCagaUACUGCaaCGagag -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 107047 | 0.68 | 0.969085 |
Target: 5'- uGGCACCACGU--GUGGCGagugGCUa-- -3' miRNA: 3'- -CCGUGGUGCAgaUACUGCaa--CGAgag -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 65060 | 0.68 | 0.965917 |
Target: 5'- cGGCACUGCGUCUAcucGGCGgcGCg--- -3' miRNA: 3'- -CCGUGGUGCAGAUa--CUGCaaCGagag -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 90046 | 0.7 | 0.932181 |
Target: 5'- uGGCACCGCGgggugcgUUAUGcACGUgggccauggccgUGUUCUCc -3' miRNA: 3'- -CCGUGGUGCa------GAUAC-UGCA------------ACGAGAG- -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 87411 | 0.74 | 0.752989 |
Target: 5'- aGGCACCACGcuUCUGUGaAUGUgGUUCUg -3' miRNA: 3'- -CCGUGGUGC--AGAUAC-UGCAaCGAGAg -5' |
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29140 | 3' | -51.5 | NC_006146.1 | + | 157750 | 1.12 | 0.005738 |
Target: 5'- cGGCACCACGUCUAUGACGUUGCUCUCc -3' miRNA: 3'- -CCGUGGUGCAGAUACUGCAACGAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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