Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29144 | 3' | -54.5 | NC_006146.1 | + | 4810 | 0.69 | 0.816481 |
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Target: 5'- aCUGuCAGGCUCcuccaGCaca-CCAGCAGCa -3' miRNA: 3'- -GAC-GUCCGAGa----CGaaaaGGUCGUCGa -5' |
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| 29144 | 3' | -54.5 | NC_006146.1 | + | 20317 | 0.69 | 0.789021 |
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Target: 5'- cCUGCAGGCgggUGCUggacgauggCCAGCAGa- -3' miRNA: 3'- -GACGUCCGag-ACGAaaa------GGUCGUCga -5' |
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| 29144 | 3' | -54.5 | NC_006146.1 | + | 115593 | 0.72 | 0.668417 |
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Target: 5'- cCUGuCAGGCUucgccCUGCacuUUUUCCGGCuGCUg -3' miRNA: 3'- -GAC-GUCCGA-----GACG---AAAAGGUCGuCGA- -5' |
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| 29144 | 3' | -54.5 | NC_006146.1 | + | 50339 | 0.74 | 0.553294 |
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Target: 5'- uCUGacCAGGCUCUGgUUUUCCAGCccguggagauccAGCg -3' miRNA: 3'- -GAC--GUCCGAGACgAAAAGGUCG------------UCGa -5' |
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| 29144 | 3' | -54.5 | NC_006146.1 | + | 13551 | 0.66 | 0.938522 |
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Target: 5'- gCUGCAGGCgccgggcgccgggCUGCgcaagcaggCCGGgGGCUc -3' miRNA: 3'- -GACGUCCGa------------GACGaaaa-----GGUCgUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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