Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29153 | 3' | -49.7 | NC_006146.1 | + | 150684 | 0.69 | 0.986086 |
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Target: 5'- gGGGAguggCCAGGGGGacggAGGGccugGCcUGCg -3' miRNA: 3'- -CCCUa---GGUUUCUCa---UCCCua--CGuACG- -5' |
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| 29153 | 3' | -49.7 | NC_006146.1 | + | 84718 | 0.68 | 0.989205 |
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Target: 5'- aGGGAcUgGAGGAGgagcucauUAGGGAUG-AUGCa -3' miRNA: 3'- -CCCUaGgUUUCUC--------AUCCCUACgUACG- -5' |
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| 29153 | 3' | -49.7 | NC_006146.1 | + | 71456 | 0.68 | 0.990545 |
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Target: 5'- uGGcgCCGAGGAGcgAGGGccuCAUGCu -3' miRNA: 3'- cCCuaGGUUUCUCa-UCCCuacGUACG- -5' |
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| 29153 | 3' | -49.7 | NC_006146.1 | + | 81655 | 0.68 | 0.99175 |
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Target: 5'- uGGGGcUCGGAGGGUgccGGGGAaGCG-GCg -3' miRNA: 3'- -CCCUaGGUUUCUCA---UCCCUaCGUaCG- -5' |
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| 29153 | 3' | -49.7 | NC_006146.1 | + | 141588 | 0.68 | 0.993795 |
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Target: 5'- gGGGAgggAGAGAGgagGGGGAgGCggGCg -3' miRNA: 3'- -CCCUaggUUUCUCa--UCCCUaCGuaCG- -5' |
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| 29153 | 3' | -49.7 | NC_006146.1 | + | 144666 | 0.68 | 0.993795 |
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Target: 5'- gGGGAgggAGAGAGgagGGGGAgGCggGCg -3' miRNA: 3'- -CCCUaggUUUCUCa--UCCCUaCGuaCG- -5' |
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| 29153 | 3' | -49.7 | NC_006146.1 | + | 147743 | 0.68 | 0.993795 |
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Target: 5'- gGGGAgggAGAGAGgagGGGGAgGCggGCg -3' miRNA: 3'- -CCCUaggUUUCUCa--UCCCUaCGuaCG- -5' |
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| 29153 | 3' | -49.7 | NC_006146.1 | + | 33240 | 0.66 | 0.998571 |
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Target: 5'- uGGGGUUCcGGGGGUGGGGGcuccacgaacccGCuUGCc -3' miRNA: 3'- -CCCUAGGuUUCUCAUCCCUa-----------CGuACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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