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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29156 | 3' | -56.6 | NC_006146.1 | + | 3568 | 0.68 | 0.822151 |
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Target: 5'- gCAgCCgaGCGUGACGAcgggacgccccgccGGCCCCccCUg -3' miRNA: 3'- aGUgGGaaCGCACUGCU--------------UCGGGGa-GA- -5' |
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| 29156 | 3' | -56.6 | NC_006146.1 | + | 24451 | 0.69 | 0.742956 |
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Target: 5'- gCGCCCgcGUGUGugGAccGCCCaCUCg -3' miRNA: 3'- aGUGGGaaCGCACugCUu-CGGG-GAGa -5' |
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| 29156 | 3' | -56.6 | NC_006146.1 | + | 157453 | 0.69 | 0.733281 |
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Target: 5'- cCACCCggGC-UGcCGggGUCCCUCc -3' miRNA: 3'- aGUGGGaaCGcACuGCuuCGGGGAGa -5' |
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| 29156 | 3' | -56.6 | NC_006146.1 | + | 154375 | 0.69 | 0.733281 |
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Target: 5'- cCACCCggGC-UGcCGggGUCCCUCc -3' miRNA: 3'- aGUGGGaaCGcACuGCuuCGGGGAGa -5' |
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| 29156 | 3' | -56.6 | NC_006146.1 | + | 151297 | 0.69 | 0.733281 |
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Target: 5'- cCACCCggGC-UGcCGggGUCCCUCc -3' miRNA: 3'- aGUGGGaaCGcACuGCuuCGGGGAGa -5' |
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| 29156 | 3' | -56.6 | NC_006146.1 | + | 148219 | 0.69 | 0.733281 |
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Target: 5'- cCACCCggGC-UGcCGggGUCCCUCc -3' miRNA: 3'- aGUGGGaaCGcACuGCuuCGGGGAGa -5' |
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| 29156 | 3' | -56.6 | NC_006146.1 | + | 145141 | 0.69 | 0.733281 |
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Target: 5'- cCACCCggGC-UGcCGggGUCCCUCc -3' miRNA: 3'- aGUGGGaaCGcACuGCuuCGGGGAGa -5' |
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| 29156 | 3' | -56.6 | NC_006146.1 | + | 142063 | 0.69 | 0.733281 |
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Target: 5'- cCACCCggGC-UGcCGggGUCCCUCc -3' miRNA: 3'- aGUGGGaaCGcACuGCuuCGGGGAGa -5' |
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| 29156 | 3' | -56.6 | NC_006146.1 | + | 111847 | 0.7 | 0.69381 |
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Target: 5'- gUCGCCC-UGCGUGuCGGcccgcuGCgCCCUCa -3' miRNA: 3'- -AGUGGGaACGCACuGCUu-----CG-GGGAGa -5' |
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| 29156 | 3' | -56.6 | NC_006146.1 | + | 129693 | 0.66 | 0.892644 |
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Target: 5'- gCGCCCagUGCccgcgGAUGggGCgCCUCa -3' miRNA: 3'- aGUGGGa-ACGca---CUGCuuCGgGGAGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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