Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29163 | 3' | -58.9 | NC_006150.1 | + | 218391 | 0.69 | 0.689564 |
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Target: 5'- aGCGGGCGGAgGGCaugcuGCCuGGUUuGCCGc -3' miRNA: 3'- gCGCUCGUUUgUCG-----CGGuCCGA-CGGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 888 | 0.69 | 0.722384 |
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Target: 5'- aGCGAGCugaaggAAccgguuuuuauuucaGCGuGCGCCGGGC-GCCGg -3' miRNA: 3'- gCGCUCG------UU---------------UGU-CGCGGUCCGaCGGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 38106 | 0.68 | 0.728098 |
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Target: 5'- gCGCGAauGCGG--GGCGCU-GGCUGCCu -3' miRNA: 3'- -GCGCU--CGUUugUCGCGGuCCGACGGc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 210572 | 0.68 | 0.73756 |
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Target: 5'- aCGCuuGAGUAGACuGCGCaCcuGCUGCCu -3' miRNA: 3'- -GCG--CUCGUUUGuCGCG-GucCGACGGc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 80502 | 0.68 | 0.73756 |
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Target: 5'- uCGCGAGcCAGACAGUGUgAGcgUGCCa -3' miRNA: 3'- -GCGCUC-GUUUGUCGCGgUCcgACGGc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 1051 | 0.68 | 0.73756 |
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Target: 5'- uCGCGgauaguugauAGUAGGCGGCGCgCAGGCaGgCGa -3' miRNA: 3'- -GCGC----------UCGUUUGUCGCG-GUCCGaCgGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 183874 | 0.68 | 0.746936 |
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Target: 5'- uGgGAGCGgugguugcAGCGGCGCaggcaaCGGGCUGCgGa -3' miRNA: 3'- gCgCUCGU--------UUGUCGCG------GUCCGACGgC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 764 | 1.09 | 0.002078 |
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Target: 5'- gCGCGAGCAAACAGCGCCAGGCUGCCGg -3' miRNA: 3'- -GCGCUCGUUUGUCGCGGUCCGACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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