Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29163 | 3' | -58.9 | NC_006150.1 | + | 122609 | 0.69 | 0.6798 |
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Target: 5'- cCGCGAGCgGGACGGCGUuuauuagauCAuugugugacucuGGCUGCCu -3' miRNA: 3'- -GCGCUCG-UUUGUCGCG---------GU------------CCGACGGc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 170813 | 0.71 | 0.581385 |
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Target: 5'- gGCGAGUAAuacugcACGGgGUCAGGCggGCCc -3' miRNA: 3'- gCGCUCGUU------UGUCgCGGUCCGa-CGGc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 149657 | 0.71 | 0.581385 |
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Target: 5'- aCGCGAuGgAAGCAGcCGCCAaaGCUGCCu -3' miRNA: 3'- -GCGCU-CgUUUGUC-GCGGUc-CGACGGc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 88406 | 0.72 | 0.542594 |
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Target: 5'- uGCGGugcGCGGACGgccGCGCUGGGuCUGCCGc -3' miRNA: 3'- gCGCU---CGUUUGU---CGCGGUCC-GACGGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 32698 | 0.72 | 0.542594 |
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Target: 5'- gCGUGGGCA-ACAGCGUCgcucgcacgAGGgaGCCGg -3' miRNA: 3'- -GCGCUCGUuUGUCGCGG---------UCCgaCGGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 213776 | 0.72 | 0.523513 |
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Target: 5'- uGCuGGCAAACAGCGCgCAauaaaaaauGGCUGUCa -3' miRNA: 3'- gCGcUCGUUUGUCGCG-GU---------CCGACGGc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 119270 | 0.72 | 0.523513 |
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Target: 5'- gCGCGuGGCcAGCGGCGCCGGaaUGCCc -3' miRNA: 3'- -GCGC-UCGuUUGUCGCGGUCcgACGGc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 764 | 1.09 | 0.002078 |
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Target: 5'- gCGCGAGCAAACAGCGCCAGGCUGCCGg -3' miRNA: 3'- -GCGCUCGUUUGUCGCGGUCCGACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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