miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
29164 3' -53 NC_006150.1 + 76101 0.66 0.989495
Target:  5'- aGguGCCACGGUGUucgaacccgaGGUGGGg-UAUUAc -3'
miRNA:   3'- -UguCGGUGUCACA----------CCGCCCaaAUAGU- -5'
29164 3' -53 NC_006150.1 + 87932 0.66 0.988085
Target:  5'- cGCAGCCuCGGUGggggGGCGGaGccgguUCAa -3'
miRNA:   3'- -UGUCGGuGUCACa---CCGCC-Caaau-AGU- -5'
29164 3' -53 NC_006150.1 + 88382 0.66 0.986533
Target:  5'- cCAGcCCGCGGcGgGGUGGGgUUGUCGa -3'
miRNA:   3'- uGUC-GGUGUCaCaCCGCCCaAAUAGU- -5'
29164 3' -53 NC_006150.1 + 40896 0.67 0.978734
Target:  5'- uACuGCCACcGUGUGGCGGa------- -3'
miRNA:   3'- -UGuCGGUGuCACACCGCCcaaauagu -5'
29164 3' -53 NC_006150.1 + 152398 0.67 0.978734
Target:  5'- gGCGGCCACGGccugcgggGUGuGCGGGacUGUUu -3'
miRNA:   3'- -UGUCGGUGUCa-------CAC-CGCCCaaAUAGu -5'
29164 3' -53 NC_006150.1 + 142078 0.67 0.976345
Target:  5'- -aGGUguCAGUGaGGCGGGcgUAUCGu -3'
miRNA:   3'- ugUCGguGUCACaCCGCCCaaAUAGU- -5'
29164 3' -53 NC_006150.1 + 4559 1.09 0.00761
Target:  5'- gACAGCCACAGUGUGGCGGGUUUAUCAu -3'
miRNA:   3'- -UGUCGGUGUCACACCGCCCAAAUAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.