Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29164 | 3' | -53 | NC_006150.1 | + | 76101 | 0.66 | 0.989495 |
Target: 5'- aGguGCCACGGUGUucgaacccgaGGUGGGg-UAUUAc -3' miRNA: 3'- -UguCGGUGUCACA----------CCGCCCaaAUAGU- -5' |
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29164 | 3' | -53 | NC_006150.1 | + | 87932 | 0.66 | 0.988085 |
Target: 5'- cGCAGCCuCGGUGggggGGCGGaGccgguUCAa -3' miRNA: 3'- -UGUCGGuGUCACa---CCGCC-Caaau-AGU- -5' |
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29164 | 3' | -53 | NC_006150.1 | + | 88382 | 0.66 | 0.986533 |
Target: 5'- cCAGcCCGCGGcGgGGUGGGgUUGUCGa -3' miRNA: 3'- uGUC-GGUGUCaCaCCGCCCaAAUAGU- -5' |
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29164 | 3' | -53 | NC_006150.1 | + | 40896 | 0.67 | 0.978734 |
Target: 5'- uACuGCCACcGUGUGGCGGa------- -3' miRNA: 3'- -UGuCGGUGuCACACCGCCcaaauagu -5' |
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29164 | 3' | -53 | NC_006150.1 | + | 152398 | 0.67 | 0.978734 |
Target: 5'- gGCGGCCACGGccugcgggGUGuGCGGGacUGUUu -3' miRNA: 3'- -UGUCGGUGUCa-------CAC-CGCCCaaAUAGu -5' |
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29164 | 3' | -53 | NC_006150.1 | + | 142078 | 0.67 | 0.976345 |
Target: 5'- -aGGUguCAGUGaGGCGGGcgUAUCGu -3' miRNA: 3'- ugUCGguGUCACaCCGCCCaaAUAGU- -5' |
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29164 | 3' | -53 | NC_006150.1 | + | 4559 | 1.09 | 0.00761 |
Target: 5'- gACAGCCACAGUGUGGCGGGUUUAUCAu -3' miRNA: 3'- -UGUCGGUGUCACACCGCCCAAAUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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