Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29166 | 3' | -53.3 | NC_006150.1 | + | 169025 | 0.7 | 0.905941 |
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Target: 5'- cCCGuGAUCACCGUgGgGUCAguCGGGGGCa -3' miRNA: 3'- -GGUcUUAGUGGCAgU-CGGU--GUCCUCG- -5' |
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| 29166 | 3' | -53.3 | NC_006150.1 | + | 88429 | 0.72 | 0.857739 |
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Target: 5'- cCCAGAAggGCgGgggcgcgCGGCC-CAGGAGCc -3' miRNA: 3'- -GGUCUUagUGgCa------GUCGGuGUCCUCG- -5' |
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| 29166 | 3' | -53.3 | NC_006150.1 | + | 86604 | 0.72 | 0.856975 |
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Target: 5'- cCCAGAAUCACCGUCGaaucugcGCC-CGacGGCa -3' miRNA: 3'- -GGUCUUAGUGGCAGU-------CGGuGUccUCG- -5' |
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| 29166 | 3' | -53.3 | NC_006150.1 | + | 92467 | 0.72 | 0.842098 |
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Target: 5'- aUCAGAAaCACCGUCacAGCgGCgAGGAGg -3' miRNA: 3'- -GGUCUUaGUGGCAG--UCGgUG-UCCUCg -5' |
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| 29166 | 3' | -53.3 | NC_006150.1 | + | 65786 | 0.75 | 0.71428 |
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Target: 5'- gCCAGGAUggaguugCACCGUCAGgccaCCACGGcaGAGCg -3' miRNA: 3'- -GGUCUUA-------GUGGCAGUC----GGUGUC--CUCG- -5' |
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| 29166 | 3' | -53.3 | NC_006150.1 | + | 90824 | 0.78 | 0.544911 |
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Target: 5'- uCCAGcacacAUCACCGcggguGCCGCAGGAGCc -3' miRNA: 3'- -GGUCu----UAGUGGCagu--CGGUGUCCUCG- -5' |
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| 29166 | 3' | -53.3 | NC_006150.1 | + | 205896 | 0.81 | 0.414794 |
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Target: 5'- aCCGGggUCGCCGUCuuuGCCugggaccuccuCAGGGGUg -3' miRNA: 3'- -GGUCuuAGUGGCAGu--CGGu----------GUCCUCG- -5' |
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| 29166 | 3' | -53.3 | NC_006150.1 | + | 23608 | 1.13 | 0.004476 |
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Target: 5'- cCCAGAAUCACCGUCAGCCACAGGAGCc -3' miRNA: 3'- -GGUCUUAGUGGCAGUCGGUGUCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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