Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29166 | 5' | -59.9 | NC_006150.1 | + | 91145 | 0.66 | 0.806432 |
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Target: 5'- aGGCAUCgGUGUauccuggGGCuCAUCGUCUUu -3' miRNA: 3'- aCCGUAGaCACGga-----CCG-GUAGCGGGA- -5' |
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| 29166 | 5' | -59.9 | NC_006150.1 | + | 117860 | 0.66 | 0.797819 |
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Target: 5'- aGGCGUCUG-GCUaucccGGCCAU-GCUCUc -3' miRNA: 3'- aCCGUAGACaCGGa----CCGGUAgCGGGA- -5' |
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| 29166 | 5' | -59.9 | NC_006150.1 | + | 165413 | 0.66 | 0.780181 |
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Target: 5'- gGGUAUCaucaucuacUGUGCUUcuaCCGUCGCCCUc -3' miRNA: 3'- aCCGUAG---------ACACGGAcc-GGUAGCGGGA- -5' |
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| 29166 | 5' | -59.9 | NC_006150.1 | + | 83866 | 0.66 | 0.771173 |
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Target: 5'- cUGGCAaaaggUCUGUGCCaacgCAUUGCCCa -3' miRNA: 3'- -ACCGU-----AGACACGGaccgGUAGCGGGa -5' |
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| 29166 | 5' | -59.9 | NC_006150.1 | + | 190059 | 0.67 | 0.752817 |
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Target: 5'- aGGCugaUUGUGCUUGGCCuGUUGgCCg -3' miRNA: 3'- aCCGua-GACACGGACCGG-UAGCgGGa -5' |
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| 29166 | 5' | -59.9 | NC_006150.1 | + | 118682 | 0.67 | 0.72456 |
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Target: 5'- aGGCGUCUcUGCCccGGCCAcgacgguaacuUUGUCCUc -3' miRNA: 3'- aCCGUAGAcACGGa-CCGGU-----------AGCGGGA- -5' |
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| 29166 | 5' | -59.9 | NC_006150.1 | + | 156668 | 0.69 | 0.626775 |
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Target: 5'- aGGU-UCUuguacuccaGUGCCUGGUgGUCGCUCUg -3' miRNA: 3'- aCCGuAGA---------CACGGACCGgUAGCGGGA- -5' |
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| 29166 | 5' | -59.9 | NC_006150.1 | + | 191719 | 0.69 | 0.616895 |
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Target: 5'- cGGCuugUUGUugccuGCCUGGUUcUCGCCCUg -3' miRNA: 3'- aCCGua-GACA-----CGGACCGGuAGCGGGA- -5' |
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| 29166 | 5' | -59.9 | NC_006150.1 | + | 23573 | 1.07 | 0.002209 |
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Target: 5'- aUGGCAUCUGUGCCUGGCCAUCGCCCUu -3' miRNA: 3'- -ACCGUAGACACGGACCGGUAGCGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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