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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29167 | 3' | -46.6 | NC_006150.1 | + | 49574 | 0.66 | 0.999969 |
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Target: 5'- uUUCUGAGccGCGCgaaucUCCCgucGCGCAGc -3' miRNA: 3'- uAAGACUU--UGUGau---AGGGaa-UGCGUU- -5' |
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| 29167 | 3' | -46.6 | NC_006150.1 | + | 202339 | 0.66 | 0.999969 |
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Target: 5'- aAUUCUGG--UACUAucauUCCgUUGCGCAAc -3' miRNA: 3'- -UAAGACUuuGUGAU----AGGgAAUGCGUU- -5' |
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| 29167 | 3' | -46.6 | NC_006150.1 | + | 129630 | 0.66 | 0.999958 |
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Target: 5'- cUUCgUGggGCGCgagGUCCCU-ACGUg- -3' miRNA: 3'- uAAG-ACuuUGUGa--UAGGGAaUGCGuu -5' |
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| 29167 | 3' | -46.6 | NC_006150.1 | + | 67849 | 0.67 | 0.999924 |
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Target: 5'- -aUCUG-AACACUGgagaCCCaUUAUGCAGa -3' miRNA: 3'- uaAGACuUUGUGAUa---GGG-AAUGCGUU- -5' |
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| 29167 | 3' | -46.6 | NC_006150.1 | + | 146936 | 0.67 | 0.999924 |
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Target: 5'- cAUUCUGAuGACgGCUAUCgCCUUAUGUc- -3' miRNA: 3'- -UAAGACU-UUG-UGAUAG-GGAAUGCGuu -5' |
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| 29167 | 3' | -46.6 | NC_006150.1 | + | 77091 | 0.69 | 0.998924 |
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Target: 5'- -gUCUGAGACACUggccagaagAUCCCgaa-GCAGg -3' miRNA: 3'- uaAGACUUUGUGA---------UAGGGaaugCGUU- -5' |
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| 29167 | 3' | -46.6 | NC_006150.1 | + | 79305 | 0.79 | 0.822794 |
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Target: 5'- cUUCUGGAGCAUUGUCUCUuccauagUACGCAAu -3' miRNA: 3'- uAAGACUUUGUGAUAGGGA-------AUGCGUU- -5' |
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| 29167 | 3' | -46.6 | NC_006150.1 | + | 26674 | 1.05 | 0.044748 |
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Target: 5'- cAUUCUGAAACACUAUCCCUUACGCAAa -3' miRNA: 3'- -UAAGACUUUGUGAUAGGGAAUGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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