Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29168 | 5' | -58.9 | NC_006150.1 | + | 27250 | 0.66 | 0.851698 |
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Target: 5'- --uCCGgcg-GCCACUCCUACCUGGUc -3' miRNA: 3'- uacGGCacagCGGUGAGGAUGGGCUA- -5' |
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| 29168 | 5' | -58.9 | NC_006150.1 | + | 183596 | 0.68 | 0.748415 |
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Target: 5'- -gGCUGUGUCGUCucuuuCUCCgcaGCCCGu- -3' miRNA: 3'- uaCGGCACAGCGGu----GAGGa--UGGGCua -5' |
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| 29168 | 5' | -58.9 | NC_006150.1 | + | 114024 | 0.69 | 0.651187 |
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Target: 5'- cUG-CGUGUCGCUACaCCUGCCgGAUu -3' miRNA: 3'- uACgGCACAGCGGUGaGGAUGGgCUA- -5' |
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| 29168 | 5' | -58.9 | NC_006150.1 | + | 88107 | 0.7 | 0.631315 |
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Target: 5'- uAUGCCcagGUCGCCACUggcgaUUGCCCGGa -3' miRNA: 3'- -UACGGca-CAGCGGUGAg----GAUGGGCUa -5' |
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| 29168 | 5' | -58.9 | NC_006150.1 | + | 126602 | 0.71 | 0.542695 |
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Target: 5'- cGUGCUGUa-CGCCACUCCggGCCUGGUu -3' miRNA: 3'- -UACGGCAcaGCGGUGAGGa-UGGGCUA- -5' |
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| 29168 | 5' | -58.9 | NC_006150.1 | + | 45266 | 1.04 | 0.004358 |
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Target: 5'- aAUGCCGUGUCGCCACUCCUACCCGAUg -3' miRNA: 3'- -UACGGCACAGCGGUGAGGAUGGGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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