Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29169 | 3' | -50.4 | NC_006150.1 | + | 123930 | 0.66 | 0.99929 |
Target: 5'- --cUCACCUGAGAGUUgaGGCUGauaaagGUGg -3' miRNA: 3'- ccuAGUGGACUUUCAG--CUGACa-----CGCa -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 155663 | 0.66 | 0.99929 |
Target: 5'- gGGAUUcagACCUGucccuguGGUacUGugUGUGCGUg -3' miRNA: 3'- -CCUAG---UGGACuu-----UCA--GCugACACGCA- -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 4213 | 0.66 | 0.99929 |
Target: 5'- uGGUCACCgu-AGGcCuGCUGUGCGg -3' miRNA: 3'- cCUAGUGGacuUUCaGcUGACACGCa -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 28250 | 0.66 | 0.998466 |
Target: 5'- aGAUCACCUGAAcauaucugaAGU--GCgGUGCGUu -3' miRNA: 3'- cCUAGUGGACUU---------UCAgcUGaCACGCA- -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 62778 | 0.67 | 0.996946 |
Target: 5'- aGGuAUCuuaCUGAgcGUCGugUGUGCa- -3' miRNA: 3'- -CC-UAGug-GACUuuCAGCugACACGca -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 92460 | 0.68 | 0.995809 |
Target: 5'- uGGAUCGCUUGAucGggGGCgUGUGCu- -3' miRNA: 3'- -CCUAGUGGACUuuCagCUG-ACACGca -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 220793 | 0.68 | 0.994348 |
Target: 5'- aGGAUgCAUUgcaGAGGUCGGCUGaUGCGg -3' miRNA: 3'- -CCUA-GUGGac-UUUCAGCUGAC-ACGCa -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 121687 | 0.69 | 0.988864 |
Target: 5'- uGGAUCAUUUGggGcggaaugcuaauGUCguGACUGUGUGUc -3' miRNA: 3'- -CCUAGUGGACuuU------------CAG--CUGACACGCA- -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 149911 | 0.7 | 0.983982 |
Target: 5'- uGGGUC-UCUGGAAGcCGGCcacgGUGCGa -3' miRNA: 3'- -CCUAGuGGACUUUCaGCUGa---CACGCa -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 69758 | 0.71 | 0.96319 |
Target: 5'- gGGcAUCgcuGCCUGcgGGUCGGCUGgaagGCGg -3' miRNA: 3'- -CC-UAG---UGGACuuUCAGCUGACa---CGCa -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 129591 | 0.72 | 0.955912 |
Target: 5'- cGAUCGCCUcaaaGAGGUgcugaaGGCUGUGCGUg -3' miRNA: 3'- cCUAGUGGAc---UUUCAg-----CUGACACGCA- -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 12778 | 0.74 | 0.898913 |
Target: 5'- cGGuaGUCACCUGAGGGgccggCGGCUGUGg-- -3' miRNA: 3'- -CC--UAGUGGACUUUCa----GCUGACACgca -5' |
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29169 | 3' | -50.4 | NC_006150.1 | + | 59780 | 1.05 | 0.02671 |
Target: 5'- aGAUCACCUGAAAGUCGACUGUGCGUu -3' miRNA: 3'- cCUAGUGGACUUUCAGCUGACACGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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