Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29170 | 3' | -55.9 | NC_006150.1 | + | 295 | 0.66 | 0.964242 |
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Target: 5'- cGCCGUCGUgccgaggccgCGGaggacggcugCCCGgggUUGGCGGu -3' miRNA: 3'- uCGGCAGUAa---------GCUa---------GGGCa--GACCGCC- -5' |
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| 29170 | 3' | -55.9 | NC_006150.1 | + | 148382 | 0.66 | 0.960945 |
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Target: 5'- gGGCCGUUccgCG-UCCgCGgauggugCUGGCGGu -3' miRNA: 3'- -UCGGCAGuaaGCuAGG-GCa------GACCGCC- -5' |
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| 29170 | 3' | -55.9 | NC_006150.1 | + | 70055 | 0.67 | 0.92693 |
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Target: 5'- uGGCagCGUCA-UCGAacUCCCGcUCUGGUGc -3' miRNA: 3'- -UCG--GCAGUaAGCU--AGGGC-AGACCGCc -5' |
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| 29170 | 3' | -55.9 | NC_006150.1 | + | 75819 | 0.69 | 0.864021 |
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Target: 5'- gAGCUGUCA--CGAUCCCGUgaagcgcaugcugCUGGauaaGGa -3' miRNA: 3'- -UCGGCAGUaaGCUAGGGCA-------------GACCg---CC- -5' |
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| 29170 | 3' | -55.9 | NC_006150.1 | + | 30270 | 0.76 | 0.533023 |
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Target: 5'- gAGUCGUCAUUUcccugGGUCCCGUCUGGa-- -3' miRNA: 3'- -UCGGCAGUAAG-----CUAGGGCAGACCgcc -5' |
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| 29170 | 3' | -55.9 | NC_006150.1 | + | 71259 | 1.1 | 0.004641 |
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Target: 5'- gAGCCGUCAUUCGAUCCCGUCUGGCGGc -3' miRNA: 3'- -UCGGCAGUAAGCUAGGGCAGACCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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