Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29171 | 3' | -46.7 | NC_006150.1 | + | 183105 | 0.66 | 0.999996 |
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Target: 5'- cGGGGugg-CGAAAGUcggGGCCGGUggUCGa -3' miRNA: 3'- -CCUCuuaaGUUUUCA---CCGGUCAa-GGC- -5' |
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| 29171 | 3' | -46.7 | NC_006150.1 | + | 102828 | 0.67 | 0.999961 |
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Target: 5'- cGAGAguuuAUUCAAcAAGUGaGgUAGUUCCGu -3' miRNA: 3'- cCUCU----UAAGUU-UUCAC-CgGUCAAGGC- -5' |
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| 29171 | 3' | -46.7 | NC_006150.1 | + | 183909 | 0.69 | 0.999601 |
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Target: 5'- aGAGAGUU---AAG-GGUCGGUUCCGc -3' miRNA: 3'- cCUCUUAAguuUUCaCCGGUCAAGGC- -5' |
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| 29171 | 3' | -46.7 | NC_006150.1 | + | 180794 | 0.69 | 0.999502 |
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Target: 5'- gGGAGGAacCGAAGGUaGCCAGgUCCu -3' miRNA: 3'- -CCUCUUaaGUUUUCAcCGGUCaAGGc -5' |
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| 29171 | 3' | -46.7 | NC_006150.1 | + | 218595 | 0.72 | 0.994528 |
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Target: 5'- aGGAGGGggaCAAGgugugucGGUGGUCAGUUCuCGg -3' miRNA: 3'- -CCUCUUaa-GUUU-------UCACCGGUCAAG-GC- -5' |
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| 29171 | 3' | -46.7 | NC_006150.1 | + | 23212 | 0.73 | 0.991683 |
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Target: 5'- aGGAGAGUUCAGGAGaGGCguGguugUuuGg -3' miRNA: 3'- -CCUCUUAAGUUUUCaCCGguCa---AggC- -5' |
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| 29171 | 3' | -46.7 | NC_006150.1 | + | 72557 | 1.14 | 0.017955 |
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Target: 5'- cGGAGAAUUCAAAAGUGGCCAGUUCCGa -3' miRNA: 3'- -CCUCUUAAGUUUUCACCGGUCAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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