Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29171 | 5' | -61.5 | NC_006150.1 | + | 88550 | 0.66 | 0.764065 |
Target: 5'- gCGCGGuuccCUGGUCGCaGCC-AGGCUu -3' miRNA: 3'- -GCGUCuu--GACCGGCGaCGGcUCCGGc -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 74621 | 0.66 | 0.761378 |
Target: 5'- uGCAGAAC-GGUcacaucgaucuucaCGCUGCUGAGgGUCa -3' miRNA: 3'- gCGUCUUGaCCG--------------GCGACGGCUC-CGGc -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 150129 | 0.66 | 0.745983 |
Target: 5'- gGCGGAugcauagacgACUGG-UGCcGCCGAGGCg- -3' miRNA: 3'- gCGUCU----------UGACCgGCGaCGGCUCCGgc -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 112889 | 0.66 | 0.745983 |
Target: 5'- gGCGGAAUuuugUGGCUguuGCUGCUGuGGuuGu -3' miRNA: 3'- gCGUCUUG----ACCGG---CGACGGCuCCggC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 71104 | 0.67 | 0.718213 |
Target: 5'- uGCAGGACggcgGGaCCuaGCUGCUGuaguggcGGCCGu -3' miRNA: 3'- gCGUCUUGa---CC-GG--CGACGGCu------CCGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 117905 | 0.67 | 0.718213 |
Target: 5'- uCGguGAGuCUGGCCaCaGCCG-GGUCGg -3' miRNA: 3'- -GCguCUU-GACCGGcGaCGGCuCCGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 113111 | 0.67 | 0.708815 |
Target: 5'- gGCGGAaaGCcGGCCGCgaUUGAGGCUGu -3' miRNA: 3'- gCGUCU--UGaCCGGCGacGGCUCCGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 837 | 0.67 | 0.699359 |
Target: 5'- uGUAGacgGugUGGCCGCgagcGCUGAGucaGCCGg -3' miRNA: 3'- gCGUC---UugACCGGCGa---CGGCUC---CGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 92174 | 0.67 | 0.689852 |
Target: 5'- aGCAuGugUcGGuuGCUGCuCGuGGCCGg -3' miRNA: 3'- gCGUcUugA-CCggCGACG-GCuCCGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 150172 | 0.67 | 0.689852 |
Target: 5'- cCGC-GAACUGuGUCGCa-CCGuGGCCGg -3' miRNA: 3'- -GCGuCUUGAC-CGGCGacGGCuCCGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 90749 | 0.67 | 0.679346 |
Target: 5'- gCGUGGGccacacaACgGGuuGCUGCgGAGGCUGc -3' miRNA: 3'- -GCGUCU-------UGaCCggCGACGgCUCCGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 156671 | 0.68 | 0.661107 |
Target: 5'- uGCAGAAgUGGuCCGCaagacUGCaGAGGCUu -3' miRNA: 3'- gCGUCUUgACC-GGCG-----ACGgCUCCGGc -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 174894 | 0.68 | 0.645688 |
Target: 5'- aCGcCAGAAgUGGCgcugcucauucaaucUGCcGUCGAGGCCGc -3' miRNA: 3'- -GC-GUCUUgACCG---------------GCGaCGGCUCCGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 216664 | 0.68 | 0.638932 |
Target: 5'- uGCAGGggucgcugcugcugACUGGuuGCUcGCCGuGGCgGc -3' miRNA: 3'- gCGUCU--------------UGACCggCGA-CGGCuCCGgC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 70092 | 0.68 | 0.632175 |
Target: 5'- uGCAcGGACUGGCgguggCGCUGCgugGAGGCUu -3' miRNA: 3'- gCGU-CUUGACCG-----GCGACGg--CUCCGGc -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 130769 | 0.68 | 0.632175 |
Target: 5'- aGCAGGagccGCUGcuGCUGCUGCUGccGCCGc -3' miRNA: 3'- gCGUCU----UGAC--CGGCGACGGCucCGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 119098 | 0.68 | 0.612874 |
Target: 5'- uCGUcGAACuugaUGGCgugCGCaGCCGAGGCCa -3' miRNA: 3'- -GCGuCUUG----ACCG---GCGaCGGCUCCGGc -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 171813 | 0.69 | 0.584036 |
Target: 5'- gCGuCAuuGCUGGCUGCUGUCGcucacGGGCUGu -3' miRNA: 3'- -GC-GUcuUGACCGGCGACGGC-----UCCGGC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 181282 | 0.7 | 0.518068 |
Target: 5'- gGCGGGAgaGGCgGCUGCCcuGGGCgGc -3' miRNA: 3'- gCGUCUUgaCCGgCGACGGc-UCCGgC- -5' |
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29171 | 5' | -61.5 | NC_006150.1 | + | 147359 | 0.7 | 0.518068 |
Target: 5'- gGCAcGGCaaaaGCCGCgGCCGAGGCUGa -3' miRNA: 3'- gCGUcUUGac--CGGCGaCGGCUCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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