miRNA: 3'- aGUGCUa----GGGCaCUUCgC-GUACGACG- -5'
miRNA: 3'- -AGUGCu--AGGGCaCUUCGCGUAcgACG- -5'
miRNA: 3'- -AGUGCUA-GGgCACUUCG-CGu------------ACGACG- -5'
miRNA: 3'- agUGCUAG-----GGCACuuCGCGuaCGACG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29172 | 5' | -53.7 | NC_006150.1 | + | 168967 | 0.67 | 0.974638 |
|
Target: 5'- cCAUGAgccacCCCG-GAGG-GaCAUGCUGCu -3' miRNA: 3'- aGUGCUa----GGGCaCUUCgC-GUACGACG- -5' |
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| 29172 | 5' | -53.7 | NC_006150.1 | + | 199102 | 0.66 | 0.985277 |
|
Target: 5'- aUUACGccuUCUCGgGAGGCGCAUuaUGUu -3' miRNA: 3'- -AGUGCu--AGGGCaCUUCGCGUAcgACG- -5' |
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| 29172 | 5' | -53.7 | NC_006150.1 | + | 213430 | 0.67 | 0.970847 |
|
Target: 5'- gUUACGAUgCUgGUGAGGCuGCuugcgguaguguuUGCUGCu -3' miRNA: 3'- -AGUGCUA-GGgCACUUCG-CGu------------ACGACG- -5' |
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| 29172 | 5' | -53.7 | NC_006150.1 | + | 216091 | 0.66 | 0.979412 |
|
Target: 5'- -uGCGGUCgauggCUGUGggGCGUcaGCUGUc -3' miRNA: 3'- agUGCUAG-----GGCACuuCGCGuaCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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