Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29172 | 5' | -53.7 | NC_006150.1 | + | 49584 | 0.66 | 0.979412 |
Target: 5'- gCGCGAaucUCCCGUc--GCGCA-GCaUGCa -3' miRNA: 3'- aGUGCU---AGGGCAcuuCGCGUaCG-ACG- -5' |
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29172 | 5' | -53.7 | NC_006150.1 | + | 135976 | 0.66 | 0.979412 |
Target: 5'- -gGCGuagCuCCGUGcuGGCacGCGUGCUGCu -3' miRNA: 3'- agUGCua-G-GGCACu-UCG--CGUACGACG- -5' |
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29172 | 5' | -53.7 | NC_006150.1 | + | 29482 | 0.66 | 0.981533 |
Target: 5'- -uGCGggCCCGgGGAGCGUacAUGCcgaGCg -3' miRNA: 3'- agUGCuaGGGCaCUUCGCG--UACGa--CG- -5' |
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29172 | 5' | -53.7 | NC_006150.1 | + | 139348 | 0.72 | 0.825142 |
Target: 5'- aUCACGGUCuuGUGuGGCGUuuguuacgggagGUGCgcugGCg -3' miRNA: 3'- -AGUGCUAGggCACuUCGCG------------UACGa---CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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