Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29174 | 3' | -47.1 | NC_006150.1 | + | 96426 | 0.66 | 0.999976 |
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Target: 5'- gUGuGAUUGUGGCUacggUUCCCGGuaaccaaaUGACGg -3' miRNA: 3'- -ACuCUAGUAUUGGa---AAGGGCU--------ACUGC- -5' |
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| 29174 | 3' | -47.1 | NC_006150.1 | + | 47480 | 0.67 | 0.999957 |
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Target: 5'- -cGGAUCAUAGCgauuugUUUgCCGAUGugGa -3' miRNA: 3'- acUCUAGUAUUGg-----AAAgGGCUACugC- -5' |
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| 29174 | 3' | -47.1 | NC_006150.1 | + | 131334 | 0.67 | 0.999957 |
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Target: 5'- cUGGGAUC-UGACCUcggUUCCGAgcuaucGGCGc -3' miRNA: 3'- -ACUCUAGuAUUGGAa--AGGGCUa-----CUGC- -5' |
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| 29174 | 3' | -47.1 | NC_006150.1 | + | 32139 | 0.7 | 0.998792 |
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Target: 5'- gUGAGAUggccCGUGACCagagUUCCCauuguccuggugGAUGGCGg -3' miRNA: 3'- -ACUCUA----GUAUUGGa---AAGGG------------CUACUGC- -5' |
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| 29174 | 3' | -47.1 | NC_006150.1 | + | 105521 | 0.71 | 0.995188 |
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Target: 5'- cGGGAcugcUCAUuuCCUUUCCCGAagaucUGGCc -3' miRNA: 3'- aCUCU----AGUAuuGGAAAGGGCU-----ACUGc -5' |
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| 29174 | 3' | -47.1 | NC_006150.1 | + | 87461 | 1.1 | 0.028703 |
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Target: 5'- cUGAGAUCAUAACCUUUCCCGAUGACGu -3' miRNA: 3'- -ACUCUAGUAUUGGAAAGGGCUACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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