Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29174 | 5' | -47.3 | NC_006150.1 | + | 42238 | 0.66 | 0.999971 |
Target: 5'- gGCGUCAgaugCcgAAucGGuGGUuaGUGUGGCa -3' miRNA: 3'- aUGCAGUa---GuaUUu-UC-CCA--CACACCG- -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 12908 | 0.66 | 0.99996 |
Target: 5'- gUGCGUUGUCGUugu-GGGUuuUGUGGUu -3' miRNA: 3'- -AUGCAGUAGUAuuuuCCCAc-ACACCG- -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 32907 | 0.67 | 0.99993 |
Target: 5'- uUGCGaCGUCc----AGGGUccuGUGUGGCa -3' miRNA: 3'- -AUGCaGUAGuauuuUCCCA---CACACCG- -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 123417 | 0.67 | 0.999879 |
Target: 5'- gAUGUUGUUcUGAucGGGcGUGUGGUa -3' miRNA: 3'- aUGCAGUAGuAUUuuCCCaCACACCG- -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 153067 | 0.69 | 0.999038 |
Target: 5'- cGCG-CAUCGUAcGAGGGUGgggGaUGGa -3' miRNA: 3'- aUGCaGUAGUAUuUUCCCACa--C-ACCg -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 160532 | 0.69 | 0.999038 |
Target: 5'- cUGCGUCAUCAauUGGcauAGGGccaugGUG-GGCa -3' miRNA: 3'- -AUGCAGUAGU--AUUu--UCCCa----CACaCCG- -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 124319 | 0.69 | 0.999038 |
Target: 5'- cGCGUCAggugucuaCAUAAAA-GGUGagGUGGCu -3' miRNA: 3'- aUGCAGUa-------GUAUUUUcCCACa-CACCG- -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 93738 | 0.7 | 0.997493 |
Target: 5'- -uCGUCGUCAUAGAagucaGGGGUGagGUuuuugcaGGCa -3' miRNA: 3'- auGCAGUAGUAUUU-----UCCCACa-CA-------CCG- -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 180452 | 0.7 | 0.997074 |
Target: 5'- gACGUCGUCGUAGccgugcgacgcGGGGGaaUGcggcgcgGUGGCg -3' miRNA: 3'- aUGCAGUAGUAUU-----------UUCCC--ACa------CACCG- -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 148420 | 0.82 | 0.660594 |
Target: 5'- --aGUC-UCAUAGcaguGGGGGUGUGUGGCa -3' miRNA: 3'- augCAGuAGUAUU----UUCCCACACACCG- -5' |
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29174 | 5' | -47.3 | NC_006150.1 | + | 87427 | 1.12 | 0.017256 |
Target: 5'- cUACGUCAUCAUAAAAGGGUGUGUGGCa -3' miRNA: 3'- -AUGCAGUAGUAUUUUCCCACACACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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