Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29175 | 3' | -55.4 | NC_006150.1 | + | 3602 | 0.66 | 0.971483 |
Target: 5'- cCUGUGCaaUGucaCCCGUUUUCCGGGAa- -3' miRNA: 3'- -GACACGccAC---GGGUAGAAGGCCUUgg -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 87443 | 0.66 | 0.971483 |
Target: 5'- --aUGCGGUGCCCuuaCUUCaauauaguuaaaUGGGACg -3' miRNA: 3'- gacACGCCACGGGua-GAAG------------GCCUUGg -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 198049 | 0.66 | 0.971483 |
Target: 5'- uCUGUGCaGUGCCCA----CgGGcGCCa -3' miRNA: 3'- -GACACGcCACGGGUagaaGgCCuUGG- -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 79292 | 0.66 | 0.968634 |
Target: 5'- ---aGCGGaUGCgCAaCUUCUGGAGCa -3' miRNA: 3'- gacaCGCC-ACGgGUaGAAGGCCUUGg -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 48427 | 0.66 | 0.958884 |
Target: 5'- gCUGUGCGucuccuaucGUGUCUGUgaUUCCGGuAACCc -3' miRNA: 3'- -GACACGC---------CACGGGUAg-AAGGCC-UUGG- -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 53880 | 0.66 | 0.955217 |
Target: 5'- cCUGUGCaacacGUGCCC----UCgGGAACCu -3' miRNA: 3'- -GACACGc----CACGGGuagaAGgCCUUGG- -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 88371 | 0.67 | 0.942908 |
Target: 5'- gCUGUGCGGUcCCCggGUUggCCGGGuauGCUc -3' miRNA: 3'- -GACACGCCAcGGG--UAGaaGGCCU---UGG- -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 173833 | 0.68 | 0.923356 |
Target: 5'- uCUG-GaCGGUGUCaugCUUaCCGGAACCc -3' miRNA: 3'- -GACaC-GCCACGGguaGAA-GGCCUUGG- -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 46359 | 0.69 | 0.887258 |
Target: 5'- gUG-GCGGUGCUCAUCggaaaacaagUCCGGGc-- -3' miRNA: 3'- gACaCGCCACGGGUAGa---------AGGCCUugg -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 24521 | 0.69 | 0.873479 |
Target: 5'- gUGUGCGGUGCauaCGgc-UCUGGAuCCa -3' miRNA: 3'- gACACGCCACGg--GUagaAGGCCUuGG- -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 73192 | 0.72 | 0.718739 |
Target: 5'- gUGUGCGGUGCCCAUgcaUUCCcaguucgaGGAuauAUCa -3' miRNA: 3'- gACACGCCACGGGUAg--AAGG--------CCU---UGG- -5' |
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29175 | 3' | -55.4 | NC_006150.1 | + | 87659 | 1.11 | 0.003605 |
Target: 5'- aCUGUGCGGUGCCCAUCUUCCGGAACCc -3' miRNA: 3'- -GACACGCCACGGGUAGAAGGCCUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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