Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29175 | 5' | -48.8 | NC_006150.1 | + | 215047 | 0.66 | 0.99975 |
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Target: 5'- -gGGCUUCAGAGc-CGGUGUGgAUc- -3' miRNA: 3'- ugCUGAAGUCUUuuGCCACACgUGua -5' |
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| 29175 | 5' | -48.8 | NC_006150.1 | + | 91494 | 0.67 | 0.999237 |
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Target: 5'- gGCGGCcguUUCuGGAgcuccaGAGCGGUGUcGCGCGUg -3' miRNA: 3'- -UGCUG---AAG-UCU------UUUGCCACA-CGUGUA- -5' |
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| 29175 | 5' | -48.8 | NC_006150.1 | + | 87408 | 0.67 | 0.999237 |
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Target: 5'- gGCGGgUUcCGGAAGAUGGgcaccgcacaGUGCGCAUg -3' miRNA: 3'- -UGCUgAA-GUCUUUUGCCa---------CACGUGUA- -5' |
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| 29175 | 5' | -48.8 | NC_006150.1 | + | 81422 | 0.68 | 0.997158 |
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Target: 5'- gGCGGCggCAGAGGG-GGUGgGCGCGg -3' miRNA: 3'- -UGCUGaaGUCUUUUgCCACaCGUGUa -5' |
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| 29175 | 5' | -48.8 | NC_006150.1 | + | 124782 | 0.69 | 0.995375 |
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Target: 5'- uGCGACcUCGGGAGcgGGUGUGCucuCGUg -3' miRNA: 3'- -UGCUGaAGUCUUUugCCACACGu--GUA- -5' |
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| 29175 | 5' | -48.8 | NC_006150.1 | + | 72346 | 0.76 | 0.858487 |
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Target: 5'- gGCGGCUUCAGGuacuuGACGaGgGUGCGCGUg -3' miRNA: 3'- -UGCUGAAGUCUu----UUGC-CaCACGUGUA- -5' |
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| 29175 | 5' | -48.8 | NC_006150.1 | + | 87625 | 1.07 | 0.025302 |
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Target: 5'- cACGACUUCAGAAAACGGUGUGCACAUg -3' miRNA: 3'- -UGCUGAAGUCUUUUGCCACACGUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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