Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29176 | 3' | -66.5 | NC_006150.1 | + | 150241 | 0.67 | 0.459551 |
Target: 5'- gAGCGCcagcggggugGCGUUCgGCaCCCUGCCcGGGc -3' miRNA: 3'- -UCGCG----------UGCGAGgCGgGGGGCGGuCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 182090 | 0.67 | 0.511352 |
Target: 5'- aGGCGaaCAUGUUCgGCCCgaaCCCGCUGGGc -3' miRNA: 3'- -UCGC--GUGCGAGgCGGG---GGGCGGUCCc -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 204911 | 0.67 | 0.502541 |
Target: 5'- -aCGgaGCGUUuuGaCCCUCGCCAGGGg -3' miRNA: 3'- ucGCg-UGCGAggCgGGGGGCGGUCCC- -5' |
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29176 | 3' | -66.5 | NC_006150.1 | + | 206388 | 0.66 | 0.538153 |
Target: 5'- uGCGCGCGCUCC-UCCCCCaUCAa-- -3' miRNA: 3'- uCGCGUGCGAGGcGGGGGGcGGUccc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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