Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29176 | 5' | -56.1 | NC_006150.1 | + | 125524 | 0.66 | 0.954599 |
Target: 5'- -aCCCUGGGGAuccguUGUGCGgaacagaGGCUAuCGg -3' miRNA: 3'- gaGGGACCUCUu----GCGCGUa------CCGAU-GC- -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 111167 | 0.66 | 0.954216 |
Target: 5'- -aCUCUGGAauGGguucaagGCGCGUGUGGCgACGg -3' miRNA: 3'- gaGGGACCU--CU-------UGCGCGUACCGaUGC- -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 112994 | 0.67 | 0.927726 |
Target: 5'- uUCUggaCUGGGGuuagaGACGCGCAUGGggGCu -3' miRNA: 3'- gAGG---GACCUC-----UUGCGCGUACCgaUGc -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 149238 | 0.68 | 0.886104 |
Target: 5'- uUCCCgUGGAGGAgGCGgCG-GGCgACGa -3' miRNA: 3'- gAGGG-ACCUCUUgCGC-GUaCCGaUGC- -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 88259 | 0.68 | 0.872243 |
Target: 5'- -gCCCUGGcggggggcGGAGCGUGCGcucGGCUggGCGg -3' miRNA: 3'- gaGGGACC--------UCUUGCGCGUa--CCGA--UGC- -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 144063 | 0.68 | 0.867923 |
Target: 5'- uCUCCCucgaUGGGGAugGCGCAacuuacuuuaaaugGGCuUAUGg -3' miRNA: 3'- -GAGGG----ACCUCUugCGCGUa-------------CCG-AUGC- -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 147692 | 0.68 | 0.865003 |
Target: 5'- --aCC-GGAGAACGCuGCuuuUGGCUGCc -3' miRNA: 3'- gagGGaCCUCUUGCG-CGu--ACCGAUGc -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 3393 | 0.69 | 0.856808 |
Target: 5'- --aCCUGGuGGACGCGUucgacacgccuugGUGGCgUGCGu -3' miRNA: 3'- gagGGACCuCUUGCGCG-------------UACCG-AUGC- -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 82207 | 0.69 | 0.842106 |
Target: 5'- gUCCCcGGAGAGCGgGCuggaGGuCUGCu -3' miRNA: 3'- gAGGGaCCUCUUGCgCGua--CC-GAUGc -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 203680 | 0.69 | 0.834102 |
Target: 5'- -gCCCcGGGGAACGUGUaucgaGUGGCUGa- -3' miRNA: 3'- gaGGGaCCUCUUGCGCG-----UACCGAUgc -5' |
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29176 | 5' | -56.1 | NC_006150.1 | + | 88470 | 1.08 | 0.004945 |
Target: 5'- gCUCCCUGGAGAACGCGCAUGGCUACGc -3' miRNA: 3'- -GAGGGACCUCUUGCGCGUACCGAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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