Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29177 | 3' | -57.8 | NC_006150.1 | + | 118355 | 0.7 | 0.712021 |
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Target: 5'- aGCAg-GGCUuaCACCACGGugGAAAa -3' miRNA: 3'- cUGUagCCGGcgGUGGUGCCugCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 120781 | 0.67 | 0.860553 |
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Target: 5'- cGCAgCGGCaCGCCACUAguCGG-CGAAAg -3' miRNA: 3'- cUGUaGCCG-GCGGUGGU--GCCuGCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 128451 | 0.68 | 0.795528 |
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Target: 5'- aGAUggCGGCUuuGCCACCAUGGaaggcgacGCGAAAc -3' miRNA: 3'- -CUGuaGCCGG--CGGUGGUGCC--------UGCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 130019 | 0.66 | 0.901388 |
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Target: 5'- uGACGUCGGUCaCgGCCGCgGGACa--- -3' miRNA: 3'- -CUGUAGCCGGcGgUGGUG-CCUGcuuu -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 130668 | 0.66 | 0.901388 |
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Target: 5'- aACGUCugaggGGCgcagGCUGCCACGGGCGAc- -3' miRNA: 3'- cUGUAG-----CCGg---CGGUGGUGCCUGCUuu -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 139386 | 0.71 | 0.623295 |
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Target: 5'- uGGCGgaCGGuCCGCCGCCugGGguACGGGAg -3' miRNA: 3'- -CUGUa-GCC-GGCGGUGGugCC--UGCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 140726 | 0.66 | 0.89511 |
|
Target: 5'- uGACcuuGUCGacgauCCGCUcgaucuuaGCCGCGGACGAGAg -3' miRNA: 3'- -CUG---UAGCc----GGCGG--------UGGUGCCUGCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 171802 | 0.66 | 0.874993 |
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Target: 5'- aGCcaaGGCUGCCAUCAUGGACa--- -3' miRNA: 3'- cUGuagCCGGCGGUGGUGCCUGcuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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