Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29177 | 5' | -57 | NC_006150.1 | + | 96279 | 0.66 | 0.921689 |
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Target: 5'- -aUCCggUGUCUggGGuUGGUGGCAc- -3' miRNA: 3'- gcAGGuuGCAGGugCC-ACCACCGUca -5' |
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| 29177 | 5' | -57 | NC_006150.1 | + | 83086 | 0.67 | 0.916226 |
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Target: 5'- --gUgGACGgcugCCA-GGUGGUGGCAGa -3' miRNA: 3'- gcaGgUUGCa---GGUgCCACCACCGUCa -5' |
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| 29177 | 5' | -57 | NC_006150.1 | + | 129375 | 0.67 | 0.904035 |
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Target: 5'- uGUgCAAUGcauaaucucgcacUCCACaaGGUGGUGGUAGa -3' miRNA: 3'- gCAgGUUGC-------------AGGUG--CCACCACCGUCa -5' |
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| 29177 | 5' | -57 | NC_006150.1 | + | 120744 | 0.67 | 0.892179 |
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Target: 5'- gGUCgGGCaGaCgACGGUGGUGGgGGUg -3' miRNA: 3'- gCAGgUUG-CaGgUGCCACCACCgUCA- -5' |
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| 29177 | 5' | -57 | NC_006150.1 | + | 89837 | 0.67 | 0.892179 |
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Target: 5'- gCGaCCGAuCGUCgACGGcuGUGGCAGUg -3' miRNA: 3'- -GCaGGUU-GCAGgUGCCacCACCGUCA- -5' |
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| 29177 | 5' | -57 | NC_006150.1 | + | 35530 | 0.67 | 0.88563 |
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Target: 5'- cCGaCCAGCG-CCACGGUcacGGU-GCGGUa -3' miRNA: 3'- -GCaGGUUGCaGGUGCCA---CCAcCGUCA- -5' |
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| 29177 | 5' | -57 | NC_006150.1 | + | 135532 | 0.71 | 0.728098 |
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Target: 5'- uCG-CCAugGUgccgCCGCGGUGGUggacgucgaGGCGGUg -3' miRNA: 3'- -GCaGGUugCA----GGUGCCACCA---------CCGUCA- -5' |
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| 29177 | 5' | -57 | NC_006150.1 | + | 148385 | 0.78 | 0.336884 |
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Target: 5'- cCGUUCcGCGUCCGCGGaUGGUgcuGGCGGUg -3' miRNA: 3'- -GCAGGuUGCAGGUGCC-ACCA---CCGUCA- -5' |
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| 29177 | 5' | -57 | NC_006150.1 | + | 88988 | 1.08 | 0.004399 |
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Target: 5'- cCGUCCAACGUCCACGGUGGUGGCAGUa -3' miRNA: 3'- -GCAGGUUGCAGGUGCCACCACCGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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