Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29178 | 3' | -56.4 | NC_006150.1 | + | 71052 | 0.66 | 0.946137 |
Target: 5'- aGGAGGAgccgcguaucagugcGGCGCaAGGggUGcGCUCGCUg -3' miRNA: 3'- aCUUUCU---------------CCGCGaUCCa-AC-CGGGCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 171091 | 0.66 | 0.935211 |
Target: 5'- gUGAgGAGuGGUGgUAuGGaccaaagGGCCCGCCu -3' miRNA: 3'- -ACU-UUCuCCGCgAU-CCaa-----CCGGGCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 157400 | 0.66 | 0.935211 |
Target: 5'- cUGAGGGuGGUGCUGGGaucuugGGCuuGg- -3' miRNA: 3'- -ACUUUCuCCGCGAUCCaa----CCGggCgg -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 48847 | 0.67 | 0.919707 |
Target: 5'- ----cGGGGUGCUAGGUUGcuUCCGUa -3' miRNA: 3'- acuuuCUCCGCGAUCCAACc-GGGCGg -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 38103 | 0.67 | 0.914082 |
Target: 5'- cGAAugcGGGGCGCUGGcugccucucgcUUGGCCCcCCc -3' miRNA: 3'- aCUUu--CUCCGCGAUCc----------AACCGGGcGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 56883 | 0.67 | 0.906431 |
Target: 5'- cGAAuaAGGCGuCUAGGcgGGCguuaccccaaacaaCCGCCu -3' miRNA: 3'- aCUUucUCCGC-GAUCCaaCCG--------------GGCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 108980 | 0.67 | 0.900286 |
Target: 5'- cGGguGAGAGGCGCc-GGUUGGCguaguugcacgcguCCGUg -3' miRNA: 3'- aCU--UUCUCCGCGauCCAACCG--------------GGCGg -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 117676 | 0.67 | 0.889332 |
Target: 5'- cGAuucGGGGGGUGCacaGGGUggcGGUCuCGCCa -3' miRNA: 3'- aCU---UUCUCCGCGa--UCCAa--CCGG-GCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 113919 | 0.67 | 0.889332 |
Target: 5'- aGAGGGAGGCaUUucGGGaccgcggGGCCuCGCCa -3' miRNA: 3'- aCUUUCUCCGcGA--UCCaa-----CCGG-GCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 39266 | 0.68 | 0.882595 |
Target: 5'- ----cGAGGgcucguguguguCGUUGGGcUGGCCCGCUc -3' miRNA: 3'- acuuuCUCC------------GCGAUCCaACCGGGCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 149133 | 0.68 | 0.845817 |
Target: 5'- gUGAGAGuGGCGCggaugaGGCCUGCg -3' miRNA: 3'- -ACUUUCuCCGCGauccaaCCGGGCGg -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 101917 | 0.68 | 0.845817 |
Target: 5'- cGAGAGAGG-GCUGcg-UGGCuuGCUg -3' miRNA: 3'- aCUUUCUCCgCGAUccaACCGggCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 143802 | 0.69 | 0.829766 |
Target: 5'- -aGAAGAGGCGCcgcuGcUGGCgCGCCg -3' miRNA: 3'- acUUUCUCCGCGauc-CaACCGgGCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 596 | 0.69 | 0.813026 |
Target: 5'- gGAGGGAGGgGgaAGGgccgcguguucUUGGCagaCCGCCa -3' miRNA: 3'- aCUUUCUCCgCgaUCC-----------AACCG---GGCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 88703 | 0.7 | 0.749914 |
Target: 5'- cGucGGA-GCGCUAGGgcGGCCCGg- -3' miRNA: 3'- aCuuUCUcCGCGAUCCaaCCGGGCgg -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 182296 | 0.71 | 0.691847 |
Target: 5'- uUGAGAGAcGGC-C-GGGUaaccGGCCCGCCc -3' miRNA: 3'- -ACUUUCU-CCGcGaUCCAa---CCGGGCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 314 | 0.72 | 0.652068 |
Target: 5'- cGGAGGAcGGCuGCccgGGGUUGGCgguCUGCCa -3' miRNA: 3'- aCUUUCU-CCG-CGa--UCCAACCG---GGCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 139374 | 0.74 | 0.542797 |
Target: 5'- cGGGAGGuGCGCUGGcGgacGGUCCGCCg -3' miRNA: 3'- aCUUUCUcCGCGAUC-Caa-CCGGGCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 89637 | 0.75 | 0.523445 |
Target: 5'- gUGAAucAGGCGCUAGGUU-GCagaCGCCg -3' miRNA: 3'- -ACUUucUCCGCGAUCCAAcCGg--GCGG- -5' |
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29178 | 3' | -56.4 | NC_006150.1 | + | 129635 | 0.79 | 0.305313 |
Target: 5'- ----uGGGGCGCgAGGUcccuacgUGGCCCGCCu -3' miRNA: 3'- acuuuCUCCGCGaUCCA-------ACCGGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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