Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29180 | 3' | -53.9 | NC_006150.1 | + | 138743 | 0.66 | 0.978001 |
Target: 5'- cACCGACcaugGCAUGCU-GUGCUcCAUCu-- -3' miRNA: 3'- -UGGCUG----UGUACGAcCGCGA-GUAGuua -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 75790 | 0.66 | 0.967005 |
Target: 5'- uGCUGGauaaggaGCA-GCUGGCGCUCAaggUCAc- -3' miRNA: 3'- -UGGCUg------UGUaCGACCGCGAGU---AGUua -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 58831 | 0.66 | 0.963737 |
Target: 5'- cACCGGCGCcgGCgggcucgaGGCGgUCAUgCAGg -3' miRNA: 3'- -UGGCUGUGuaCGa-------CCGCgAGUA-GUUa -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 109616 | 0.67 | 0.952597 |
Target: 5'- cGCCG-CACAcGCUGGCuaUCAUgGGa -3' miRNA: 3'- -UGGCuGUGUaCGACCGcgAGUAgUUa -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 123829 | 0.67 | 0.952597 |
Target: 5'- aGCCGAaugcgcagcaCACAUGCUGaCGUcggUCAUCAAc -3' miRNA: 3'- -UGGCU----------GUGUACGACcGCG---AGUAGUUa -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 90549 | 0.67 | 0.944011 |
Target: 5'- gGCCGuugucguCGC-UGCUGGCGC-CAUCu-- -3' miRNA: 3'- -UGGCu------GUGuACGACCGCGaGUAGuua -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 216634 | 0.68 | 0.93936 |
Target: 5'- cGCCGugGCGgcUGC-GGUGCUCggCAGg -3' miRNA: 3'- -UGGCugUGU--ACGaCCGCGAGuaGUUa -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 136200 | 0.69 | 0.906365 |
Target: 5'- cGCCGGucCAgAcucUGCUGGCGCUCuUCAc- -3' miRNA: 3'- -UGGCU--GUgU---ACGACCGCGAGuAGUua -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 142605 | 0.7 | 0.857132 |
Target: 5'- gACUGuCACG-GCUGGUGCUCAagGAUg -3' miRNA: 3'- -UGGCuGUGUaCGACCGCGAGUagUUA- -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 92120 | 0.7 | 0.857132 |
Target: 5'- aGCUGACGCA-GCUGGUuCUCGUCc-- -3' miRNA: 3'- -UGGCUGUGUaCGACCGcGAGUAGuua -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 149995 | 0.7 | 0.857132 |
Target: 5'- uGCCGaACGCcaccccGCUGGCGCUCccGUCAc- -3' miRNA: 3'- -UGGC-UGUGua----CGACCGCGAG--UAGUua -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 150105 | 0.71 | 0.824334 |
Target: 5'- cGCCGAgGCGUGCUGcUGCUCAacaCAGUg -3' miRNA: 3'- -UGGCUgUGUACGACcGCGAGUa--GUUA- -5' |
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29180 | 3' | -53.9 | NC_006150.1 | + | 92408 | 1.05 | 0.009885 |
Target: 5'- aACCGACACAUGCUGGCGCUCAUCAAUc -3' miRNA: 3'- -UGGCUGUGUACGACCGCGAGUAGUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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