Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29181 | 3' | -61.9 | NC_006150.1 | + | 88720 | 0.66 | 0.787137 |
Target: 5'- gCCGGcUgCCUACCcgacgucggagcGCUagggCGGCCCGGACg -3' miRNA: 3'- -GGCUuAgGGGUGG------------CGG----GCCGGGUCUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 181508 | 0.66 | 0.787137 |
Target: 5'- cCCGcccUCCgCGuCCGCCuCGGCaCCAcGACa -3' miRNA: 3'- -GGCuu-AGGgGU-GGCGG-GCCG-GGU-CUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 71179 | 0.66 | 0.778553 |
Target: 5'- aCGAucgCUCCGCCGCCCuuuccaccGCCCAcACc -3' miRNA: 3'- gGCUua-GGGGUGGCGGGc-------CGGGUcUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 174345 | 0.66 | 0.752146 |
Target: 5'- -----gCCCCGCCGCCCuGGCCgAa-- -3' miRNA: 3'- ggcuuaGGGGUGGCGGG-CCGGgUcug -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 172381 | 0.66 | 0.743151 |
Target: 5'- aCCGAGUCCCgcgcguaucuuuCACUcuggcaCCUGGCCCucuGGCa -3' miRNA: 3'- -GGCUUAGGG------------GUGGc-----GGGCCGGGu--CUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 110873 | 0.66 | 0.743151 |
Target: 5'- cCCGAGUUaCCCACUuacgauGCCgCuGUCCAGGCg -3' miRNA: 3'- -GGCUUAG-GGGUGG------CGG-GcCGGGUCUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 140167 | 0.66 | 0.743151 |
Target: 5'- aCGGAgaCCCACCcUCUGGCCCAaGAUc -3' miRNA: 3'- gGCUUagGGGUGGcGGGCCGGGU-CUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 71874 | 0.66 | 0.742247 |
Target: 5'- -aGGAUCCUCcccguguACCGCCCGcGCCgAG-Cg -3' miRNA: 3'- ggCUUAGGGG-------UGGCGGGC-CGGgUCuG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 132443 | 0.66 | 0.741341 |
Target: 5'- gCCGAggaguuuGUCUCCGCgGUcgcggcuCUGGCCCAGgACg -3' miRNA: 3'- -GGCU-------UAGGGGUGgCG-------GGCCGGGUC-UG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 1732 | 0.67 | 0.715691 |
Target: 5'- aCCGGcaCCgCagACCGCCuCaGCCCAGACc -3' miRNA: 3'- -GGCUuaGGgG--UGGCGG-GcCGGGUCUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 707 | 0.67 | 0.687675 |
Target: 5'- uUGAcgCCCC-CCGCCgCGaccCCCGGGCc -3' miRNA: 3'- gGCUuaGGGGuGGCGG-GCc--GGGUCUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 135785 | 0.68 | 0.659299 |
Target: 5'- cUCGAcGUCCaCCACCGCggCGGCaCCAuGGCg -3' miRNA: 3'- -GGCU-UAGG-GGUGGCGg-GCCG-GGU-CUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 118167 | 0.68 | 0.659299 |
Target: 5'- gCGAggcGUgCCgACCGaCCCGGCuguggCCAGACu -3' miRNA: 3'- gGCU---UAgGGgUGGC-GGGCCG-----GGUCUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 169796 | 0.68 | 0.630753 |
Target: 5'- -aGAcUCUgCACCGaCCCGGUgauCCAGACu -3' miRNA: 3'- ggCUuAGGgGUGGC-GGGCCG---GGUCUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 182286 | 0.68 | 0.62123 |
Target: 5'- gCCGggUaaCCGgcCCGCCCaGGUCCAGGu -3' miRNA: 3'- -GGCuuAggGGU--GGCGGG-CCGGGUCUg -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 565 | 0.7 | 0.545799 |
Target: 5'- gCGAAaCCCgaGCCGCCCGGCU--GACu -3' miRNA: 3'- gGCUUaGGGg-UGGCGGGCCGGguCUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 182333 | 0.7 | 0.509131 |
Target: 5'- cCCGAGaCCCCAUgcugUGCCCGGCCUc--- -3' miRNA: 3'- -GGCUUaGGGGUG----GCGGGCCGGGucug -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 66002 | 0.71 | 0.481442 |
Target: 5'- uUGGAUCUgcauuuccagcugCCGCUGUCCGGCCCGGuaGCg -3' miRNA: 3'- gGCUUAGG-------------GGUGGCGGGCCGGGUC--UG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 58566 | 0.72 | 0.447684 |
Target: 5'- cUCGGAUCCCUccugcaugACCGCCuCGaGCCCGccGGCg -3' miRNA: 3'- -GGCUUAGGGG--------UGGCGG-GC-CGGGU--CUG- -5' |
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29181 | 3' | -61.9 | NC_006150.1 | + | 148054 | 0.72 | 0.435889 |
Target: 5'- gCCGAGcgcagucguggugCCCuCACCGUCCaGGCaCCGGACg -3' miRNA: 3'- -GGCUUa------------GGG-GUGGCGGG-CCG-GGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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