Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29182 | 5' | -53.4 | NC_006150.1 | + | 40267 | 0.66 | 0.991659 |
Target: 5'- aUCUgGAUC---CGggCCAGGUGCGGGg -3' miRNA: 3'- -GGGgCUAGuucGCuaGGUCCAUGCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 90980 | 0.66 | 0.990513 |
Target: 5'- gUCCCGGUUGAGCGGagacacacCCgAGGcgggGCGAGg -3' miRNA: 3'- -GGGGCUAGUUCGCUa-------GG-UCCa---UGCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 182893 | 0.66 | 0.990392 |
Target: 5'- gCCUCGG-CGGGgGcUCCGGGUaguggcaGCGAGg -3' miRNA: 3'- -GGGGCUaGUUCgCuAGGUCCA-------UGCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 110666 | 0.67 | 0.982822 |
Target: 5'- gCCUgGAUgGAGUguuaGGUCggCGGGUACGAGg -3' miRNA: 3'- -GGGgCUAgUUCG----CUAG--GUCCAUGCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 69932 | 0.68 | 0.968275 |
Target: 5'- gCCCCaGAgcUCGcAGCGGUaCCAGGUACc-- -3' miRNA: 3'- -GGGG-CU--AGU-UCGCUA-GGUCCAUGcuc -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 119843 | 0.68 | 0.968275 |
Target: 5'- uCCCagGAUCAGuuugauGCGGUCacucuccucucgCAGGUGCGGGg -3' miRNA: 3'- -GGGg-CUAGUU------CGCUAG------------GUCCAUGCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 72427 | 0.68 | 0.961898 |
Target: 5'- aCCCCGAUCAAGCGu----GGUugcuCGAu -3' miRNA: 3'- -GGGGCUAGUUCGCuagguCCAu---GCUc -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 26787 | 0.68 | 0.961557 |
Target: 5'- cCCCCGcgUAAGUGGcUCCAGGcacucccUGCuGAGa -3' miRNA: 3'- -GGGGCuaGUUCGCU-AGGUCC-------AUG-CUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 88758 | 0.69 | 0.942212 |
Target: 5'- gUCCCGAUCcAGCacugcGUCCAGGgcucguCGGGg -3' miRNA: 3'- -GGGGCUAGuUCGc----UAGGUCCau----GCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 215276 | 0.7 | 0.927187 |
Target: 5'- uUCCCGGUCAGGCGuuucgcuAUCCcauAGGgacguggcgGCGAGu -3' miRNA: 3'- -GGGGCUAGUUCGC-------UAGG---UCCa--------UGCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 10047 | 0.71 | 0.898951 |
Target: 5'- cCCUCGGUCaAAGauGUCCAGGUGCa-- -3' miRNA: 3'- -GGGGCUAG-UUCgcUAGGUCCAUGcuc -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 88145 | 0.72 | 0.871941 |
Target: 5'- aCCCCGccgCGGGCugGAUCCugcGGUAUGGGg -3' miRNA: 3'- -GGGGCua-GUUCG--CUAGGu--CCAUGCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 139483 | 0.73 | 0.833586 |
Target: 5'- gCuuGaAUCAAGCGugggagagCCGGGUGCGGGa -3' miRNA: 3'- gGggC-UAGUUCGCua------GGUCCAUGCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 205495 | 0.82 | 0.344654 |
Target: 5'- gUCCCGAUCAuuaucccuguGCGGUUCAGGUAUGAGc -3' miRNA: 3'- -GGGGCUAGUu---------CGCUAGGUCCAUGCUC- -5' |
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29182 | 5' | -53.4 | NC_006150.1 | + | 92699 | 1.12 | 0.005823 |
Target: 5'- cCCCCGAUCAAGCGAUCCAGGUACGAGg -3' miRNA: 3'- -GGGGCUAGUUCGCUAGGUCCAUGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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