miRNA: 3'- uauGUgGCACCGCCUUUGGGG--CUCGU- -5'
miRNA: 3'- -UAUGUGg-----CACCGCCUUugGGGCUCGU- -5'
miRNA: 3'- -UAUGUGGCACCGCCUUUGGGGCUCGU- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29183 | 3' | -57.1 | NC_006150.1 | + | 220759 | 0.71 | 0.697403 |
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Target: 5'- --uUAgCGUGGUGGGAGCUCCggGAGCAu -3' miRNA: 3'- uauGUgGCACCGCCUUUGGGG--CUCGU- -5' |
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| 29183 | 3' | -57.1 | NC_006150.1 | + | 197716 | 0.74 | 0.500639 |
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Target: 5'- cAUGCACaugaauGUGGCGGAG--CCCGAGCGa -3' miRNA: 3'- -UAUGUGg-----CACCGCCUUugGGGCUCGU- -5' |
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| 29183 | 3' | -57.1 | NC_006150.1 | + | 92871 | 1.06 | 0.004684 |
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Target: 5'- uAUACACCGUGGCGGAAACCCCGAGCAg -3' miRNA: 3'- -UAUGUGGCACCGCCUUUGGGGCUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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