Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29185 | 5' | -60.7 | NC_006150.1 | + | 203121 | 0.66 | 0.751859 |
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Target: 5'- cGAGGCGcCCGCGuuCCAGGUgCCcgCgCUg -3' miRNA: 3'- uUUCCGC-GGCGC--GGUCCA-GGuaG-GAa -5' |
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| 29185 | 5' | -60.7 | NC_006150.1 | + | 85153 | 0.66 | 0.742478 |
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Target: 5'- aAGAGGCGCUGC-UCAaGUCCAUUCa- -3' miRNA: 3'- -UUUCCGCGGCGcGGUcCAGGUAGGaa -5' |
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| 29185 | 5' | -60.7 | NC_006150.1 | + | 119278 | 0.68 | 0.605382 |
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Target: 5'- -cAGGUGCCGCGCguGG-CCAgcggcgCCg- -3' miRNA: 3'- uuUCCGCGGCGCGguCCaGGUa-----GGaa -5' |
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| 29185 | 5' | -60.7 | NC_006150.1 | + | 116713 | 0.7 | 0.505502 |
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Target: 5'- --uGGCGuuGgGCCGGGUCCAgcugagcgcguacuUCCa- -3' miRNA: 3'- uuuCCGCggCgCGGUCCAGGU--------------AGGaa -5' |
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| 29185 | 5' | -60.7 | NC_006150.1 | + | 138973 | 0.7 | 0.477668 |
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Target: 5'- cAAGGCGCCGCcaaaugaccaggcaGCCAGuuGUCCAUCg-- -3' miRNA: 3'- uUUCCGCGGCG--------------CGGUC--CAGGUAGgaa -5' |
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| 29185 | 5' | -60.7 | NC_006150.1 | + | 117634 | 0.71 | 0.426998 |
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Target: 5'- gAAAGGCGCUggGCGCCAa-UCCGUCCg- -3' miRNA: 3'- -UUUCCGCGG--CGCGGUccAGGUAGGaa -5' |
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| 29185 | 5' | -60.7 | NC_006150.1 | + | 86419 | 0.72 | 0.404967 |
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Target: 5'- aGGGGGCGgCGCGCCAGGcgcacggugcacauuUCCGUgCUg -3' miRNA: 3'- -UUUCCGCgGCGCGGUCC---------------AGGUAgGAa -5' |
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| 29185 | 5' | -60.7 | NC_006150.1 | + | 95691 | 1.02 | 0.003577 |
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Target: 5'- cAAAGGCGCCGCGCCAGGUCCAUCCUUc -3' miRNA: 3'- -UUUCCGCGGCGCGGUCCAGGUAGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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